Trull Anna K, van der Horst Jelle, Palenstijn Willem Jan, van Vliet Lucas J, van Leeuwen Tristan, Kalkman Jeroen
Department of Imaging Physics, Delft University of Technology, Lorentzweg 1, 2628 CJ Delft, Netherlands.
Phys Med Biol. 2017 Sep 21;62(19):7784-7797. doi: 10.1088/1361-6560/aa8945.
As a result of the shallow depth of focus of the optical imaging system, the use of standard filtered back projection in optical projection tomography causes space-variant tangential blurring that increases with the distance to the rotation axis. We present a novel optical tomographic image reconstruction technique that incorporates the point spread function of the imaging lens in an iterative reconstruction. The technique is demonstrated using numerical simulations, tested on experimental optical projection tomography data of single fluorescent beads, and applied to high-resolution emission optical projection tomography imaging of an entire zebrafish larva. Compared to filtered back projection our results show greatly reduced radial and tangential blurring over the entire [Formula: see text] mm field of view, and a significantly improved signal to noise ratio.
由于光学成像系统的焦深较浅，在光学投影断层扫描中使用标准滤波反投影会导致空间变化的切向模糊，这种模糊会随着到旋转轴距离的增加而增大。我们提出了一种新颖的光学断层图像重建技术，该技术在迭代重建中纳入了成像透镜的点扩散函数。通过数值模拟对该技术进行了演示，在单个荧光珠的实验光学投影断层扫描数据上进行了测试，并应用于整个斑马鱼幼体的高分辨率发射光学投影断层扫描成像。与滤波反投影相比，我们的结果表明，在整个[公式：见正文]毫米视场内，径向和切向模糊大大降低，信噪比显著提高。
