Fathy Abdallah H, Bashandy Mohamed A, Bashandy Samir A E, Mansour Ahmed M, Elsadek Bakheet
a Department of Zoology, Faculty of Science, Al-Azhar University, Cairo, Egypt.
b Department of Pharmacology, National Research Center, Cairo, Egypt.
Can J Physiol Pharmacol. 2017 Dec;95(12):1462-1472. doi: 10.1139/cjpp-2017-0413. Epub 2017 Aug 30.
Hepatocellular carcinoma (HCC) is one of the most life-threatening cancers. The present study was designed to chronologically analyze the HCC chemically induced by diethylnitrosamine (DEN) in male Wistar rats during a 27-week period. DEN was given to rats in drinking water (100 mg/L) to induce HCC. In the present study, the DEN-administered groups recorded dramatic results in the tumor markers, oxidative stress, lipid profile, liver function, and hematological parameters at all intervals when compared with their corresponding values in the control groups. In addition, the morphometric analysis of livers of the DEN-administered groups (from 9 to 27 weeks) showed gradual enlargement and several grayish white nodules and foci on the peripheral surface of the liver as the features of HCC. In conclusion, the present sequential model chronologically analyzes all steps of hepatocarcinogenesis and presents a new staging system for classification of HCC that may be valuable for investigating the effects of anticarcinogenic compounds at varying stages of hepatocarcinogenesis in vivo.
肝细胞癌（HCC）是最具生命威胁的癌症之一。本研究旨在按时间顺序分析雄性Wistar大鼠在27周内由二乙基亚硝胺（DEN）化学诱导的HCC。将DEN加入大鼠饮用水中（100 mg/L）以诱导HCC。在本研究中，与对照组的相应值相比，DEN给药组在所有时间点的肿瘤标志物、氧化应激、血脂谱、肝功能和血液学参数方面均记录到显著结果。此外，DEN给药组（9至27周）肝脏的形态计量学分析显示肝脏逐渐肿大，肝脏外周表面有多个灰白色结节和病灶，这些是HCC的特征。总之，本序贯模型按时间顺序分析了肝癌发生的所有步骤，并提出了一种新的HCC分期系统，这对于研究体内不同肝癌发生阶段抗癌化合物的作用可能具有重要价值。
