Ko Chia-Ling, Wu Hui-Yu, Lin Yu-Sheng, Yang Chun-Hui, Chen Jian-Chih, Chen Wen-Cheng
Advanced Medical Devices and Composites Laboratory, Department of Fiber and Composite Materials, Feng Chia University. Taichung 407, Taiwan.
Dental Medical Devices and Materials Research Center, College of Dental Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
Biomed Mater Eng. 2017;28(5):515-529. doi: 10.3233/BME-171690.
A biodegradable porous particle for the controlled biofactor delivery which assembly of pores in scaffolds can improve the permeation and diffusion of drugs or growth factors.
Porous-spheres in millimeter scale were prepared by mixing sodium alginate and gelatin interpenetrating networks with cross-linkers; interconnected open pores were fabricated through solvent casting and particulate leaching.
Morphological characteristics, degradation, and bovine serum albumin (BSA) release rates of the porous-spheres immersed in three different solutions, namely, deionized distilled water, simulated body fluid (SBF), and phosphate-buffered saline (PBS), were detected.
Porous-spheres with a large amount of gelatin exhibited an increase in water absorption rates without affecting scaffold strength and no cytotoxicity was elicited. Highly interconnected pores with a diameter of 100-200 µm were uniformly distributed in scaffolds. The weight loss in PBS was faster than that in other solutions; the highest release rate of BSA in SBF was observed for 2 h. The release rates also exhibited linear patterns from 2 h to 24 h in all of the groups.
After 1 d of immersion in solutions, BSA release rates in scaffolds logarithmically decreased for 14 d. The degradation of porous-spheres also showed an inverse pattern.
一种用于生物因子可控递送的可生物降解多孔颗粒，支架中孔隙的组装可改善药物或生长因子的渗透和扩散。
通过将海藻酸钠和明胶互穿网络与交联剂混合制备毫米级多孔球；通过溶剂浇铸和颗粒沥滤制造相互连通的开孔。
检测浸入三种不同溶液（即去离子蒸馏水、模拟体液（SBF）和磷酸盐缓冲盐水（PBS））中的多孔球的形态特征、降解情况和牛血清白蛋白（BSA）释放率。
含有大量明胶的多孔球吸水率增加，且不影响支架强度，也未引发细胞毒性。直径为100 - 200 µm的高度相互连通的孔隙均匀分布在支架中。在PBS中的失重比在其他溶液中更快；在SBF中观察到BSA在2小时时释放率最高。在所有组中，释放率在2小时至24小时内也呈现线性模式。
在溶液中浸泡1天后，支架中BSA释放率在14天内呈对数下降。多孔球的降解也呈现相反模式。
