Lourenço Luís Antonio, Alberton Magina Michele Debiasi, Tavares Lorena Benathar Ballod, Guelli Ulson de Souza Selene Maria Arruda, García Román Miguel, Altmajer Vaz Deisi
a Chemical and Food Engineering Department , Federal University of Santa Catarina , Florianópolis , Brazil.
b Pharmaceutical Sciences Department , Regional University of Blumenau , Blumenau , Brazil.
Environ Technol. 2018 Dec;39(23):3066-3076. doi: 10.1080/09593330.2017.1374471. Epub 2017 Sep 16.
Biosurfactants are amphiphilic compounds of microbial origin which exhibit better properties than their chemically derived counterparts. They are usually produced in submerged fermentation by different types of bacteria. However, biosurfactant production by fungi, particularly of the white-rot type, has been scarcely studied. In this work, and for the first time, we report the production of biosurfactants by the white-rot fungus Trametes versicolor, which was grown on two-phase olive mill waste (TPOMW) in a solid-state fermentation system. The effect of the composition of the culture medium on biosurfactant production was also studied. The highest biosurfactant production (373.6 ± 19.4 mg in 100 g of culture medium) was achieved with a medium containing 35% (w/w) of TPOMW, the highest concentration used, 10% of wheat bran and 55% of olive stones. Interestingly, no inhibition of biosurfactant production by TPOMW was detected within the concentration range used (5-35% w/w). The biosurfactant produced by T. versicolor was able to reduce the surface tension of an aqueous extract of the culture medium up to 34.5 ± 0.3 mN m. A preliminary study of the chemical structure of the biosurfactant indicated that it contains both lipid and protein fractions. The simultaneous production of lignin-degrading enzymes was also assessed.
生物表面活性剂是微生物来源的两亲化合物，其性能优于化学合成的同类产品。它们通常由不同类型的细菌在深层发酵中产生。然而，真菌，特别是白腐型真菌产生生物表面活性剂的研究很少。在这项工作中，我们首次报道了白腐真菌云芝在固态发酵系统中利用两相橄榄果渣废水（TPOMW）生产生物表面活性剂。还研究了培养基组成对生物表面活性剂生产的影响。使用含有35%（w/w）TPOMW（所用最高浓度）、10%麦麸和55%橄榄石的培养基时，生物表面活性剂产量最高（100 g培养基中为373.6±19.4 mg）。有趣的是，在所使用的浓度范围（5-35% w/w）内，未检测到TPOMW对生物表面活性剂生产的抑制作用。云芝产生的生物表面活性剂能够将培养基水提取物的表面张力降低至34.5±0.3 mN m。对生物表面活性剂化学结构的初步研究表明，它含有脂质和蛋白质部分。还评估了木质素降解酶的同时产生情况。
