The Ohio State University, Department of Horticulture and Crop Science, Wooster OH, 44691, USA.
Sci Rep. 2017 Aug 30;7(1):9939. doi: 10.1038/s41598-017-09034-2.
Taraxacum kok-saghyz (TK) is a potential alternative crop for natural rubber (NR) production, due to its high molecular weight rubber, short breeding cycle, and diverse environmental adaptation. However, improvements in rubber yield and agronomically relevant traits are still required before it can become a commercially-viable crop. An RNA-Seq based transcriptome was developed from a pool of roots from genotypes with high and low rubber yield. A total of 55,532 transcripts with lengths over 200 bp were de novo assembled. As many as 472 transcripts were significantly homologous to 49 out of 50 known plant putative rubber biosynthesis related genes. 158 transcripts were significantly differentially expressed between high rubber and low rubber genotypes. 21,036 SNPs were different in high and low rubber TK genotypes. Among these, 50 SNPs were found within 39 transcripts highly homologous to 49 publically-searched rubber biosynthesis related genes. 117 SNPs were located within 36 of the differentially expressed gene sequences. This comprehensive TK transcriptomic reference, and large set of SNPs including putative exonic markers associated with rubber related gene homologues and differentially expressed genes, provides a solid foundation for further genetic dissection of rubber related traits, comparative genomics and marker-assisted selection for the breeding of TK.
蒲公英（TK）是一种有潜力的天然橡胶（NR）替代作物，因为它具有高分子量橡胶、短的繁殖周期和多样化的环境适应性。然而，在它成为一种具有商业可行性的作物之前，仍然需要提高橡胶产量和农艺相关性状。本研究从高橡胶和低橡胶产量基因型的根混合池中开发了一个基于 RNA-Seq 的转录组。总共组装了 55532 条长度超过 200bp 的转录本。多达 472 条转录本与 50 个已知的植物橡胶生物合成相关基因中的 49 个具有显著同源性。158 条转录本在高橡胶和低橡胶基因型之间差异表达显著。高橡胶和低橡胶 TK 基因型之间有 21036 个 SNP 不同。其中，在与 49 个公开搜索的橡胶生物合成相关基因高度同源的 39 个转录本中发现了 50 个 SNP。117 个 SNP 位于差异表达基因序列的 36 个中。这个全面的蒲公英转录组参考，以及包括与橡胶相关基因同源物和差异表达基因相关的假定外显子标记在内的大量 SNP，为进一步遗传剖析橡胶相关性状、比较基因组学和标记辅助选择提供了坚实的基础，以培育蒲公英。
