磷虾的脂类、脂肪酸和羟基脂肪酸。

Lipids, fatty acids and hydroxy-fatty acids of Euphausia pacifica.

机构信息

Iwate Biotechnology Research Center, 22-174-4 Narita, Kitakami, Iwate, 024-0003, Japan.

Life Science Materials Laboratory, ADEKA Corporation, 7-2-34 Higashiogu, Arakawaku, Tokyo, 116-8553, Japan.

出版信息

Sci Rep. 2017 Aug 30;7(1):9944. doi: 10.1038/s41598-017-09637-9.

Euphausia pacifica is a good candidate for a resource of marine n-3 PUFA. However, few reports exist of the lipid and fatty acid composition of E. pacifica. To examine the potential of E. pacifica as a resource of marine n-3 PUFA, we analyzed E. pacifica oil. We extracted lipids from E. pacifica harvested from the Pacific Ocean near Sanriku, Japan. Lipid classes of E. pacifica oil were analyzed by TLC-FID and the fatty acid composition of the oil was analyzed by GC/MS. Free fatty acids and hydroxy-fatty acids were analyzed by LC/QTOFMS. The lipid content of E. pacifica ranged from 1.30% to 3.57%. The ratios of triacylglycerols, phosphatidylcholine, phosphatidylethanolamine and free fatty acids in E. pacifica lipids were 5.3-23.0%, 32.6-53.4%, 8.5-25.4% and 2.5-7.0%, respectively. The content of n-3 PUFA in E. pacifica lipids was 38.6-46.5%. We also showed that E. pacifica contains unusual fatty acids and derivatives: C16-PUFAs (9,12-hexadecadienoic acid, 6,9,12-hexadecatrienoic acid and 6,9,12,15-hexadecatetraenoic acid) and hydroxy-PUFAs (8-HETE and 10-HDoHE). E. pacifica is a good resource of marine n-3 PUFA. Moreover, E. pacifica can provide C16-PUFA and hydroxy-PUFAs.

太平洋磷虾是海洋 n-3PUFA 的良好候选资源。然而，关于太平洋磷虾的脂质和脂肪酸组成的报道很少。为了研究太平洋磷虾作为海洋 n-3PUFA 资源的潜力，我们分析了太平洋磷虾油。我们从日本三陆海域捕捞的太平洋磷虾中提取了脂质。通过 TLC-FID 分析了太平洋磷虾油的脂质类别，并通过 GC/MS 分析了油的脂肪酸组成。通过 LC/QTOFMS 分析了游离脂肪酸和羟基脂肪酸。太平洋磷虾的脂质含量在 1.30%至 3.57%之间。太平洋磷虾脂质中三酰甘油、磷脂酰胆碱、磷脂酰乙醇胺和游离脂肪酸的比例分别为 5.3-23.0%、32.6-53.4%、8.5-25.4%和 2.5-7.0%。太平洋磷虾脂质中的 n-3PUFA 含量为 38.6-46.5%。我们还表明，太平洋磷虾含有不常见的脂肪酸和衍生物：C16-PUFAs（9,12-十六碳二烯酸、6,9,12-十六碳三烯酸和 6,9,12,15-十六碳四烯酸）和羟基-PUFAs（8-HETE 和 10-HDoHE）。太平洋磷虾是海洋 n-3PUFA 的良好资源。此外，太平洋磷虾可以提供 C16-PUFA 和羟基-PUFAs。