Bansal Urmil, Bariana Harbans
The University of Sydney Plant Breeding Institute, 107 Cobbitty Road, Cobbitty, NSW, 2570, Australia.
Methods Mol Biol. 2017;1659:151-162. doi: 10.1007/978-1-4939-7249-4_13.
Genetic characterisation of new rust resistance loci in wheat using cytogenetic/low-throughput genotyping systems required at least 5 years. Development of next-generation sequencing (NGS) based molecular marker genotyping platforms in the last decade has provided scientists with the genomic resources to expedite precise mapping of target loci. Here, we describe methodologies for genetic analysis and application of NGS-based resources to determine the precise genomic locations of rust resistance loci in wheat and development of closely linked markers for marker assisted selection.
使用细胞遗传学/低通量基因分型系统对小麦新的抗锈病基因座进行遗传特征分析至少需要5年时间。过去十年中基于下一代测序（NGS）的分子标记基因分型平台的发展，为科学家提供了基因组资源，以加快目标基因座的精确定位。在这里，我们描述了基于NGS的资源进行遗传分析和应用的方法，以确定小麦抗锈病基因座的精确基因组位置，并开发紧密连锁的标记用于标记辅助选择。
