Liu Qing, Liu Guocheng, Wang Ting, Fu Jing, Li Rujiao, Song Linlin, Wang Zhen-Gang, Ding Baoquan, Chen Fei
CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, Beijing, 100190, P. R. China.
University of Chinese Academy of Sciences, Beijing, 100049, P. R. China.
Chemphyschem. 2017 Nov 3;18(21):2977-2980. doi: 10.1002/cphc.201700809. Epub 2017 Sep 22.
Self-assembled DNA nanostructures hold great promise in the fields of nanofabrication, biosensing and nanomedicine. However, the inherent low stability of the DNA double helices, formed by weak interactions, largely hinders the assembly and functions of DNA nanostructures. In this study, we redesigned and constructed a six-arm DNA junction by incorporation of the unnatural base pairs 5-Me-isoC/isoG and A/2-thioT into the double helices. They not only retained the structural integrity of the DNA nanostructure, but also showed enhanced thermal stability and resistance to T7 Exonuclease digestion. This research may expand the applications of DNA nanostructures in nanofabrication and biomedical fields, and furthermore, the genetic alphabet expansion with unnatural base pairs may enable us to construct more complicated and diversified self-assembled DNA nanostructures.
自组装DNA纳米结构在纳米制造、生物传感和纳米医学领域具有巨大潜力。然而，由弱相互作用形成的DNA双螺旋固有的低稳定性在很大程度上阻碍了DNA纳米结构的组装和功能。在本研究中，我们通过将非天然碱基对5-甲基异胞嘧啶/异鸟嘌呤和腺嘌呤/2-硫代胸腺嘧啶掺入双螺旋中，重新设计并构建了一个六臂DNA连接体。它们不仅保留了DNA纳米结构的结构完整性，还表现出增强的热稳定性和对T7核酸外切酶消化的抗性。这项研究可能会扩大DNA纳米结构在纳米制造和生物医学领域的应用，此外，通过非天然碱基对进行的遗传字母扩展可能使我们能够构建更复杂、更多样化的自组装DNA纳米结构。
