基于凝胶的DNA纳米结构生物稳定性分析
Gel-Based Analysis of DNA Nanostructure Biostability.
作者信息
Chandrasekaran Arun Richard
机构信息
The RNA Institute, University at Albany, State University of New York, New York, NY, USA.
Department of Nanoscale Science and Engineering, University at Albany, State University of New York, New York, NY, USA.
出版信息
Methods Mol Biol. 2025;2901:27-34. doi: 10.1007/978-1-0716-4394-5_3.
Abstract
Biostability is a key feature for DNA nanostructures to be used in biological applications. With several strategies being employed to enhance the nuclease resistance of DNA nanostructures, methods to analyze the nuclease degradation are also needed. This chapter describes a protocol to evaluate the nuclease resistance of DNA motifs and nanostructures using polyacrylamide gel electrophoresis.
摘要
生物稳定性是DNA纳米结构用于生物应用的关键特性。由于采用了多种策略来增强DNA纳米结构的核酸酶抗性，因此也需要分析核酸酶降解的方法。本章介绍了一种使用聚丙烯酰胺凝胶电泳评估DNA基序和纳米结构核酸酶抗性的方案。
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本文引用的文献
1
Environment-Dependent Stability and Mechanical Properties of DNA Origami Six-Helix Bundles with Different Crossover Spacings.具有不同交叉间距的 DNA 折纸六螺旋束的环境依赖性稳定性和力学性能。
Small. 2022 May;18(18):e2107393. doi: 10.1002/smll.202107393. Epub 2022 Apr 1.
2
Nuclease Degradation Analysis of DNA Nanostructures Using Gel Electrophoresis.使用凝胶电泳分析核酸纳米结构的核酸酶降解。
Curr Protoc Nucleic Acid Chem. 2020 Sep;82(1):e115. doi: 10.1002/cpnc.115.
3
Exceptional Nuclease Resistance of Paranemic Crossover (PX) DNA and Crossover-Dependent Biostability of DNA Motifs.具有异常核酸酶抗性的偏分离交叉（PX）DNA 及依赖交叉的 DNA 基序的生物稳定性
J Am Chem Soc. 2020 Apr 8;142(14):6814-6821. doi: 10.1021/jacs.0c02211. Epub 2020 Mar 25.
4
DNA origami protection and molecular interfacing through engineered sequence-defined peptoids.通过工程序列定义的肽核酸实现 DNA 折纸保护和分子界面作用。
Proc Natl Acad Sci U S A. 2020 Mar 24;117(12):6339-6348. doi: 10.1073/pnas.1919749117. Epub 2020 Mar 12.
5
Glutaraldehyde Cross-Linking of Oligolysines Coating DNA Origami Greatly Reduces Susceptibility to Nuclease Degradation.寡聚赖氨酸涂层 DNA 折纸的戊二醛交联极大地降低了对核酸酶降解的敏感性。
J Am Chem Soc. 2020 Feb 19;142(7):3311-3315. doi: 10.1021/jacs.9b11698. Epub 2020 Feb 11.
6
Enhancing Biocompatible Stability of DNA Nanostructures Using Dendritic Oligonucleotides and Brick Motifs.利用树枝状寡核苷酸和积木图案增强 DNA 纳米结构的生物相容性稳定性。
Angew Chem Int Ed Engl. 2020 Jan 7;59(2):700-703. doi: 10.1002/anie.201911664. Epub 2019 Nov 19.
7
Rationally Engineered Nucleic Acid Architectures for Biosensing Applications.用于生物传感应用的理性设计核酸结构
Chem Rev. 2019 Nov 27;119(22):11631-11717. doi: 10.1021/acs.chemrev.9b00121. Epub 2019 Oct 1.
8
A Molecular Hero Suit for In Vitro and In Vivo DNA Nanostructures.一种用于体外和体内DNA纳米结构的分子“英雄套装”
Small. 2019 Jun;15(26):e1805386. doi: 10.1002/smll.201805386. Epub 2019 Apr 15.
9
The Growing Development of DNA Nanostructures for Potential Healthcare-Related Applications.DNA 纳米结构在潜在医疗相关应用中的不断发展。
Adv Healthc Mater. 2019 May;8(9):e1801546. doi: 10.1002/adhm.201801546. Epub 2019 Mar 7.
10
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Trends Biochem Sci. 2018 Dec;43(12):997-1013. doi: 10.1016/j.tibs.2018.09.010. Epub 2018 Oct 17.
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