Lee Dong Wan, Lee Aslan Hwanhwi, Lee Hanbyul, Kim Jae-Jin, Khim Jong Seong, Yim Un Hyuk, Kim Beom Seok
Division of Environmental Science and Ecological Engineering, College of Life Science and Biotechnology, Korea University, Seoul 02841, Republic of Korea.
BK21 Plus Eco-Leader Education Center, Korea University, Seoul 02841, Republic of Korea.
Int J Syst Evol Microbiol. 2017 Sep;67(9):3351-3357. doi: 10.1099/ijsem.0.002118. Epub 2017 Aug 31.
A novel Gram-stain-negative, rod-shaped, gliding bacterium, designated strain 001-Na2T, was isolated from coastal sediment of the Yellow Sea in Sindu-ri, Republic of Korea. Cells were aerobic, oxidase-positive and catalase-positive. Colonies were dark salmon-coloured, circular, and pulvinate with entire margins. 16S rRNA gene sequence analysis revealed that strain 001-Na2T belonged to the genus Echinicola and these species formed a distinct lineage within the family Cyclobacteriaceae. The closest phylogenetic neighbours were 'Echinicola shivajiensis' AK12T (98.1 % 16S rRNA gene sequence similarity), Echinicola vietnamensis LMG 23754T (96.4 %), Echinicola jeungdonensis HMD 3054T (96.0 %), Echinicola pacifica KMM 6172T (96.0 %) and Echinicola rosea JL 3085T (95.8 %). The predominant respiratory quinone was menaquinone MK-7. The major fatty acids were iso-C15 : 0, summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c), C17 : 1ω6c and summed feature 9 (comprising iso-C17 : 1ω9c and/or 10-methyl C16 : 0). The polar lipids were phosphatidylethanolamine, one unidentified aminophospholipid, one unidentified phospholipid and three unidentified lipids. The DNA G+C content was 44.6 mol%. DNA-DNA relatedness between strain 001-Na2T and 'E. shivajiensis' JCM 17847T was 20.8-21.1 %. On the basis of the phenotypic features, and phylogenetic and DNA-DNA hybridization analyses presented, strain 001-Na2T (=KCTC 52495T=JCM 31786T=DSM 103729T) is the type strain of a novel species of the genus Echinicola, for which the name Echinicola sediminis sp. nov. is proposed.
从韩国新斗里黄海沿岸沉积物中分离出一种新型革兰氏阴性、杆状、滑行细菌，命名为菌株001-Na2T。细胞为需氧型，氧化酶阳性，过氧化氢酶阳性。菌落呈深鲑鱼色，圆形，边缘完整且呈垫状。16S rRNA基因序列分析表明，菌株001-Na2T属于棘孢菌属，这些菌种在环杆菌科内形成了一个独特的谱系。最接近的系统发育邻居是“希瓦吉棘孢菌”AK12T（16S rRNA基因序列相似性为98.1%）、越南棘孢菌LMG 23754T（96.4%）、旌善棘孢菌HMD 3054T（96.0%）、太平洋棘孢菌KMM 6172T（96.0%）和玫瑰棘孢菌JL 3085T（95.8%）。主要呼吸醌为甲基萘醌MK-7。主要脂肪酸为异-C15 : 0、总和特征3（包含C16 : 1ω7c和/或C16 : 1ω6c）、C17 : 1ω6c和总和特征9（包含异-C17 : 1ω9c和/或10-甲基C16 : 0）。极性脂质为磷脂酰乙醇胺、一种未鉴定的氨基磷脂、一种未鉴定的磷脂和三种未鉴定的脂质。DNA G+C含量为44.6mol%。菌株001-Na2T与“希瓦吉棘孢菌”JCM 17847T之间的DNA-DNA相关性为20.8-21.1%。基于所呈现的表型特征、系统发育和DNA-DNA杂交分析，菌株001-Na2T（=KCTC 52495T=JCM 31786T=DSM 103729T）是棘孢菌属一个新物种的模式菌株，为此提出新名称棘孢菌属海泥种（Echinicola sediminis sp. nov.）。
