Himaman Winanda, Suksaard Paweena, Mingma Ratchanee, Matsumoto Atsuko, Duangmal Kannika
Department of Microbiology, Faculty of Science, Kasetsart University, Chatuchak, Bangkok 10900, Thailand.
Center for Advanced Studies in Tropical Natural Resources, NRU-KU, Kasetsart University, Chatuchak, Bangkok 10900, Thailand.
Int J Syst Evol Microbiol. 2017 Aug;67(8):3077-3082. doi: 10.1099/ijsem.0.002086.
A novel actinomycete, designated strain EURKPP3H10T, was isolated from surface-sterilized roots of Eucalyptus camaldulensis Dehnh., collected from Kamphaengphet Silvicultural Research Station, Kamphaengphet province, Thailand. The taxonomic position of strain EURKPP3H10T was studied using a polyphasic approach. Phylogenetic evaluation based on 16S rRNA gene sequence analysis showed that strain EURKPP3H10T belongs to the genus Cryptosporangium, with the highest sequence similarity to Cryptosporangium cibodasense LIPI11-2-Ac046T (99.2 %). Colonies of strain EURKPP3H10T were orange yellow. Spherical sporangia with motile spores were observed. The strain contained meso-diaminopimelic acid and acofriose, arabinose, galactose, glucose, mannose, xylose and ribose in whole-cell hydrolysates. The predominant menaquinones were MK-9(H8) and MK-9(H6). The major fatty acids were iso-C16 : 0, C17 : 1ω8c, C18 : 1ω9c and C17 : 0. The polar lipids of the strain were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol and unknown lipids. The DNA G+C content of the genomic DNA was 71.5 mol%. Based on comparative analysis of physiological, biochemical and chemotaxonomic data, including DNA-DNA hybridization, strain EURKPP3H10T represents a novel species of the genus Cryptosporangium, for which the name Cryptosporangium eucalypti sp. nov. is proposed. The type strain is EURKPP3H10T (=BCC 77605T=NBRC 111482T).
从泰国甘烹碧府甘烹碧造林研究站采集的经表面消毒的赤桉（Eucalyptus camaldulensis Dehnh.）根部分离出一株新的放线菌，命名为EURKPP3H10T菌株。采用多相分类法研究了EURKPP3H10T菌株的分类地位。基于16S rRNA基因序列分析的系统发育评估表明，EURKPP3H10T菌株属于隐孢囊菌属（Cryptosporangium），与西博达斯隐孢囊菌（Cryptosporangium cibodasense）LIPI11 - 2 - Ac046T的序列相似性最高（99.2%）。EURKPP3H10T菌株的菌落为橙黄色。观察到带有游动孢子的球形孢子囊。该菌株全细胞水解产物中含有内消旋二氨基庚二酸以及阿可弗糖、阿拉伯糖、半乳糖、葡萄糖、甘露糖、木糖和核糖。主要的甲基萘醌是MK - 9(H8)和MK - 9(H6)。主要脂肪酸为异C16 : 0、C17 : 1ω8c、C18 : 1ω9c和C17 : 0。该菌株的极性脂为磷脂酰乙醇胺、双磷脂酰甘油、磷脂酰肌醇和未知脂类。基因组DNA的G + C含量为71.5 mol%。基于生理、生化和化学分类数据的比较分析，包括DNA - DNA杂交，EURKPP3H10T菌株代表隐孢囊菌属的一个新物种，为此提出新名称桉木隐孢囊菌（Cryptosporangium eucalypti sp. nov.）。模式菌株为EURKPP3H10T（=BCC 77605T=NBRC 111482T）。
