Thailand Bioresource Research Center (TBRC), National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), 113 Thailand Science Park, Phaholyothin Road, Khlong Nueng, Khlong Luang, Pathum Thani, 12120, Thailand.
Int J Syst Evol Microbiol. 2020 Feb;70(2):971-976. doi: 10.1099/ijsem.0.003857.
A novel zoosporic actinomycete, designated strain A-T 5661, was isolated from plant litter collected in Thailand. Strain A-T 5661 developed large multilocular sporangia containing motile sporangiospores at the ends of sporangiophores on substrate mycelium. The results of our polyphasic taxonomic study demonstrated that the strain had characteristics typical of members of the genus . The 16S rRNA gene sequence and phylogenetic analyses indicated that strain A-T 5661 shared the sequence similarity ≤98.5 % with all members of the genus . The values of DNA-DNA relatedness that distinguished this novel strain from its closest related species were below 70 %. The cell-wall peptidoglycan contained -diaminopimelic acid. The whole-cell sugars were acofriose, arabinose, galactose, glucose, mannose, xylose and ribose. The predominant menaquinones were MK-9(H) and MK-9(H) with minor amounts of MK-9(H) and MK-9(H). The predominant fatty acids were iso-C, Cω9 and anteiso-C. The polar lipid profile consisted of phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannosides and unidentified polar lipids. The G+C content of the genomic DNA was 71.6 mol%. Based on comparative analysis of phenotypic, chemotaxonomic and genotypic data, the novel zoosporic actinomycete A-T 5661 (=TBRC 8127=NBRC 113237) is proposed to be the type strain of a novel species, sp. nov.
一株新的游动孢子放线菌，命名为 A-T 5661 菌株，从泰国收集的植物凋落物中分离得到。A-T 5661 菌株在基质菌丝上的孢梗体末端形成大的多室孢子囊，内含游动孢子囊孢子。多相分类学研究的结果表明，该菌株具有典型的属特征。16S rRNA 基因序列和系统发育分析表明，A-T 5661 菌株与属内所有成员的序列相似性≤98.5%。该新菌株与最接近的相关种区分开来的 DNA-DNA 相关值低于 70%。细胞壁肽聚糖含有二氨基庚二酸。全细胞糖为阿洛酮糖、阿拉伯糖、半乳糖、葡萄糖、甘露糖、木糖和核糖。主要的menaquinones 为 MK-9(H)和 MK-9(H)，少量的 MK-9(H)和 MK-9(H)。主要脂肪酸为 iso-C、Cω9 和 anteiso-C。极性脂谱由磷脂酰乙醇胺、双磷脂酰甘油、磷脂酰甘油、磷脂酰肌醇、磷脂酰肌醇甘露糖苷和未鉴定的极性脂组成。基因组 DNA 的 G+C 含量为 71.6 mol%。基于表型、化学分类和基因型数据的比较分析，新型游动孢子放线菌 A-T 5661(=TBRC 8127=NBRC 113237)被提议为一个新种的模式菌株，命名为 sp. nov.
