Department of Biological Sciences, University of Huddersfield, United Kingdom.
Genome Biol Evol. 2017 Aug 1;9(8):2140-2144. doi: 10.1093/gbe/evx151.
The ability of micro-organisms to degrade isosaccharinic acids (ISAs) while tolerating hyperalkaline conditions is pivotal to our understanding of the biogeochemistry associated within these environs, but also in scenarios pertaining to the cementitious disposal of radioactive wastes. An alkalitolerant, ISA degrading micro-organism was isolated from the hyperalkaline soils resulting from lime depositions. Here, we report the first whole-genome sequence, ISA degradation profile and carbohydrate preoteome of a Macellibacteroides fermentans strain HH-ZS, 4.08 Mb in size, coding 3,241 proteins, 64 tRNA, and 1 rRNA.
微生物降解异柠檬酸(ISAs)的能力,同时耐受高碱性条件,对我们理解与之相关的生物地球化学过程至关重要,这不仅包括在这些环境中,还包括与放射性废物的水泥固化处置有关的情况。我们从石灰沉积形成的高碱性土壤中分离出一种耐碱、能降解 ISA 的微生物。在这里,我们报告了第一个全基因组序列、ISA 降解谱和碳水化合物蛋白质组的马塞利伯克霍尔德菌 HH-ZS 菌株,大小为 4.08 Mb,编码 3241 种蛋白质、64 种 tRNA 和 1 种 rRNA。