Gandhi B M, Irshad M, Chawla T C, Tandon B N
Trans R Soc Trop Med Hyg. 1987;81(2):183-5. doi: 10.1016/0035-9203(87)90207-0.
Enzyme linked protein-A was used to develop an enzyme linked immunosorbent assay (ELISA) system for the detection of circulating antibodies to amoebic antigen. The specificity of protein-A to bind IgG only through Fc receptors, makes the test more specific for the detection of IgG antibodies to amoebic antigen. The ELISA system was used to detect amoebic antibody in control subjects (56), patients with amoebic liver abscess (79) and Entamoeba histolytica cyst-passers (10) and the results compared with those of indirect haemagglutination assay (IHA). The ELISA was more sensitive and detected 74.7% of cases with amoebic antibody in amoebic liver abscess compared with 66.7% detected by IHA. The test was more specific, sensitive and easy to perform and is recommended as a test of choice for the serological diagnosis of amoebic liver abscess.
使用酶联蛋白A开发了一种酶联免疫吸附测定（ELISA）系统，用于检测针对阿米巴抗原的循环抗体。蛋白A仅通过Fc受体结合IgG的特异性，使得该测试在检测针对阿米巴抗原的IgG抗体时更具特异性。该ELISA系统用于检测对照受试者（56例）、阿米巴肝脓肿患者（79例）和溶组织内阿米巴包囊携带者（10例）中的阿米巴抗体，并将结果与间接血凝试验（IHA）的结果进行比较。ELISA更敏感，在阿米巴肝脓肿中检测到74.7%的阿米巴抗体病例，而IHA检测到66.7%。该测试更具特异性、敏感性且易于操作，推荐作为阿米巴肝脓肿血清学诊断的首选测试。
