a Ministry of Agriculture Key Laboratory of Agricultural Entomology , Institute of Insect Sciences, Zhejiang University , Hangzhou , Zhejiang , PR China.
b Department of Entomology, Faculty of Veterinary and Animal Husbandry , Somali National University , Mogadishu , Somalia.
Artif Cells Nanomed Biotechnol. 2018 Sep;46(6):1171-1179. doi: 10.1080/21691401.2017.1365723. Epub 2017 Aug 31.
Mosquitoes pose a threat to humans and animals, causing millions of deaths every year. Vector control by effective eco-friendly pesticides of natural origin is a serious issue that requires urgent attention. The employment of green-reducing extracts for nanoparticles biosynthesis in a rapid and single-step process represents a promising strategy. In this study, silver nanoparticles (AgNPs) were biofabricated using an essential oil of Aquilaria sinensis (AsEO) and Pogostemonis Herba essential oil of Pogostemon cablin (PcEO) in one step and cost-effective manner. UV-vis spectrophotometry, Fourier transform infrared spectroscopy, scanning electron microscopy, transmission electron microscopy, X-ray diffraction analysis and energy-dispersive X-ray spectroscopy were used to confirm the AgNPs formation and their biophysical characterization. The larvicidal and pupicidal toxicity of AsEO, PcEO and biosynthesized AgNPs were evaluated against larvae and pupae of the dengue and Zika virus vector Aedes albopictus. Compared to the tested essential oils, the biofabricated AgNPs showed the highest toxicity against larvae and pupae of Ae.albopictus. In particular, the LC values of AsEO ranged from 44.23 (I) to 166 (pupae), LC values of PcEO ranged from 32.49 (I) to 90.05(IV), LC values of AsEO-AgNPs from 0.81 (I) to 1.12 (IV) and LC values of PcEO-AgPNs from 0.85 (I) to 1.19 (IV). Furthermore, histological analysis of the midgut cells of the control and treated larvae exhibited that the epithelial cells and brush border were highly affected by the fabricated AgNPs compared to the essential oils (AsEO and PcEO). Overall, the A. sinensis and P. cablin essential oils fabricated AgNPs have a potential of application as a biopesticide for mosquito control through safer and cost-effective approach.
蚊子对人类和动物构成威胁，每年导致数百万人死亡。通过有效的环保型天然来源农药进行病媒控制是一个亟待关注的严重问题。利用绿色还原提取物在快速单一步骤中合成纳米颗粒代表了一种有前途的策略。在这项研究中，使用沉香精油（AsEO）和藿香精油（PcEO）一步法和具有成本效益的方式生物合成了银纳米颗粒（AgNPs）。使用紫外可见分光光度法、傅里叶变换红外光谱、扫描电子显微镜、透射电子显微镜、X 射线衍射分析和能量色散 X 射线能谱法确认了 AgNPs 的形成及其物理特性。评估了沉香精油、藿香精油和生物合成的 AgNPs 对登革热和寨卡病毒载体埃及伊蚊幼虫和蛹的杀虫和杀蛹毒性。与测试的精油相比，生物合成的 AgNPs 对埃及伊蚊幼虫和蛹的毒性最高。特别是，AsEO 的 LC 值范围从 44.23（I）到 166（蛹），PcEO 的 LC 值范围从 32.49（I）到 90.05（IV），AsEO-AgNPs 的 LC 值范围从 0.81（I）到 1.12（IV），PcEO-AgPNs 的 LC 值范围从 0.85（I）到 1.19（IV）。此外，对对照和处理幼虫的中肠细胞进行组织学分析表明，与精油（AsEO 和 PcEO）相比，AgNPs 对上皮细胞和刷状缘的影响更大。总的来说，沉香和藿香精油合成的 AgNPs 具有作为生物农药控制蚊子的潜力，通过更安全和具有成本效益的方法。
