Laboratory of Parasitology, Department of Virology, Parasitology and Immunology, Faculty of Veterinary Sciences, Ghent University, Belgium.
Laboratory of Experimental Immunology, Department of Clinical Chemistry, Microbiology and Immunology, Faculty of Medicine and Health Sciences, Ghent University, Belgium.
Int J Parasitol. 2018 Jan;48(1):41-49. doi: 10.1016/j.ijpara.2017.07.002. Epub 2017 Aug 30.
The protective capacities of a native double-domain activation-associated secreted protein (ndd-ASP)-based vaccine against the cattle intestinal nematode Cooperia oncophora has previously been demonstrated. However, protection analysis upon vaccination with a recombinantly produced antigen has never been performed. Therefore, the aim of the current study was to test the protective potential of a Pichia-produced double-domain ASP (pdd-ASP)-based vaccine against C. oncophora. Additionally, we aimed to compare the cellular and humoral mechanisms underlying the vaccine-induced responses by the native (ndd-ASP) and recombinant vaccines. Immunisation of cattle with the native C. oncophora vaccine conferred significant levels of protection after an experimental challenge infection, whereas the recombinant vaccine did not. Moreover, vaccination with ndd-ASP resulted in a higher proliferation of CD4-T cells both systemically and in the small intestinal mucosa when compared with animals vaccinated with the recombinant antigen. In terms of humoral response, although both native and recombinant vaccines induced similar levels of antibodies, animals vaccinated with the native vaccine were able to raise antibodies with greater specificity towards ndd-ASP in comparison with antibodies raised by vaccination with the recombinant vaccine, suggesting a differential immune recognition towards the ndd-ASP and pdd-ASP. Finally, the observation that animals displaying antibodies with higher percentages of recognition towards ndd-ASP also exhibited the lowest egg counts suggests a potential relationship between antibody specificity and protection.
先前已经证明了一种本土双结构域激活相关分泌蛋白（ndd-ASP）为基础的疫苗对牛肠道寄生线虫旋毛虫的保护能力。然而，从未对重组抗原接种后的保护作用进行分析。因此，本研究的目的是测试毕赤酵母生产的双结构域 ASP（pdd-ASP）为基础的疫苗对旋毛虫的保护潜力。此外，我们旨在比较天然（ndd-ASP）和重组疫苗诱导的免疫反应的细胞和体液机制。用天然旋毛虫疫苗对牛进行免疫接种后，在实验性攻毒感染后产生了显著的保护水平，而重组疫苗则没有。此外，与用重组抗原接种的动物相比，用 ndd-ASP 接种导致 CD4-T 细胞在全身和小肠黏膜中的增殖更高。就体液反应而言，尽管天然和重组疫苗都诱导了相似水平的抗体，但与用重组疫苗接种相比，用天然疫苗接种的动物能够针对 ndd-ASP 产生具有更高特异性的抗体，表明对 ndd-ASP 和 pdd-ASP 的免疫识别存在差异。最后，观察到表现出针对 ndd-ASP 的更高识别百分比抗体的动物也表现出最低的卵计数，这表明抗体特异性和保护之间可能存在关系。
