Cui Yanyan, Wang Xiaoxing, Zhang Yan, Yan Yaqun, Dong Haiju, Jian Fuchun, Shi Ke, Zhang Longxian, Wang Rongjun, Ning Changshen
College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China.
Acta Trop. 2018 Jan;177:207-210. doi: 10.1016/j.actatropica.2017.08.029. Epub 2017 Aug 30.
Tick-borne diseases (TBDs) impose a significant constraint to livestock production world widely. In this paper, we presented a case of TBD in a cattle farm in Henan, China. 35 blood samples (7 samples sent by veterinarian, 28 samples gathered by our colleagues) were collected from ill, surviving and asymptomatic cattle and microscopic observation and PCR assays were conducted to characterize the pathogens. Genus Ixodes feeding on these cattle were collected and identified. Theileria annulata-like and Anaplasma marginale-like pathogens were observed in the blood smears stained with Giemsa staining under microscope. Furthermore, 5 out of 7 cattle blood samples were found to be positive for T. annulata by PCR. In the 28 blood specimens, three were positive for T. annulata, while A. marginale DNA was detected in nine blood DNA samples. Besides, 56 ticks feeding on cattle were collected from this farm and were all identified as Rhipisephalus microplus, meanwhile, 10 of them were found to be positive for A. marginale. In addition, phylogenetic analysis of the msp4 gene sequences of A. marginale obtained in this study showed that the isolate from cattle (KX840009) fell in the same clade with that of R. microplus (KX904527), sharing 100% similarity. To the best of our knowledge, this is the first confirmed report of outbreak of theileriosis/anaplasmosis in cattle farms in Henan, China.
蜱传疾病（TBDs）对全球畜牧业生产造成了重大限制。在本文中，我们介绍了中国河南一个养牛场发生的一起蜱传疾病病例。从患病、存活和无症状的牛身上采集了35份血样（7份由兽医送检，28份由我们的同事采集），并进行了显微镜观察和PCR检测以鉴定病原体。采集并鉴定了以这些牛为宿主的硬蜱属蜱虫。在吉姆萨染色的血涂片显微镜下观察到环形泰勒虫样和边缘无浆体样病原体。此外，7份牛血样中有5份经PCR检测为环形泰勒虫阳性。在28份血样中，3份为环形泰勒虫阳性，而在9份血样DNA中检测到边缘无浆体DNA。此外，从该农场采集了56只以牛为宿主的蜱虫，均鉴定为微小扇头蜱，同时其中10只被检测出边缘无浆体呈阳性。此外，对本研究中获得的边缘无浆体msp4基因序列进行系统发育分析表明，来自牛的分离株（KX840009）与微小扇头蜱的分离株（KX904527）属于同一进化枝，相似度达100%。据我们所知，这是中国河南养牛场首次确诊的泰勒虫病/无浆体病疫情报告。
