Li Tao, Cui Yanyan, Xiao Jinxiu, Jiang Yuxi, Ning Changshen, Qi Meng, Tao Dayong
College of Animal Science, Tarim University, Alar, Xinjiang, 843300, PR China.
School of Biotechnology and Food, Shangqiu Normal University, Shangqiu, 476000, PR China.
Int J Parasitol Parasites Wildl. 2021 Feb 14;14:157-160. doi: 10.1016/j.ijppaw.2021.02.008. eCollection 2021 Apr.
spp. are important zoonotic tick-borne pathogens that impact on human health. There are few reports on the prevalence and molecular genetic characteristics of Cervidae species in China. The purpose of this study, therefore, was to investigate the presence of spp. in blood samples of Tian Shan wapiti () in the Xinjiang Uygur Autonomous Region of China, and conduct phylogenetic analyses. A total of 50 blood samples (wild deer n = 26, and captive deer n = 24) were collected from the deer. PCR was used to detect spp. in the blood samples. Forty percent (20) of the samples were found to contain spp. Three species DNA were detected in deer blood samples: (n = 13), (n = 18), and (n = 11). Among the 20 spp. positive samples, 14 were mixed infection of two or three pathogens. The prevalence of species in wild deer was significantly higher than that of captive deer, 73.1% (19) vs 4.2% (1) respectively, ( < 0.01). Two sequence types (AB1, and AB2), three sequence types (AO1-AO3), and one sequence type (AP1) were obtained in this study. The sequences of AO1 shared 100% identity with a human isolate from Cyprus. Our results suggest that wild deer are more likely to become infected with spp. than captive individuals, and thus, could potentially transmit pathogens to humans.
某些物种是重要的人兽共患蜱传病原体，会影响人类健康。关于中国鹿科物种的流行情况和分子遗传特征的报道较少。因此，本研究的目的是调查在中国新疆维吾尔自治区天山马鹿血液样本中某些物种的存在情况，并进行系统发育分析。共从鹿身上采集了50份血液样本（野生鹿n = 26，圈养鹿n = 24）。采用聚合酶链反应（PCR）检测血液样本中的某些物种。发现40%（20份）的样本含有某些物种。在鹿血样本中检测到三种物种的DNA：某物种1（n = 13）、某物种2（n = 18）和某物种3（n = 11）。在20份某些物种阳性样本中，有14份是两种或三种病原体的混合感染。野生鹿中某些物种的流行率显著高于圈养鹿，分别为73.1%（19份）和4.2%（1份），（P < 0.01）。本研究获得了两种某物种序列类型（AB1和AB2）、三种某物种序列类型（AO1 - AO3）和一种某物种序列类型（AP1）。AO1的序列与来自塞浦路斯的一株人类分离株具有100%的同一性。我们的结果表明，野生鹿比圈养个体更容易感染某些物种，因此有可能将病原体传播给人类。
