Hosseini-Chegeni A, Tavakoli M, Goudarzi Gh, Telmadarraiy Z, Sharifdini M, Faghihi F, Ghanbari M K
Department of Plant Protection, Faculty of Agriculture, Lorestan University, Khorramabad, Iran.
Agricultural Research, Education and Extension Organization (AREEO), Lorestan Agricultural and Natural Resources Research Center, Khorramabad, Iran.
Arch Razi Inst. 2020 Mar;75(1):39-46. doi: 10.22092/ari.2019.122532.1259. Epub 2020 Mar 1.
The present study was conducted as the first molecular detection of Anaplasma species in tick samples based on the sequencing of major surface proteins 4 (msp4) gene fragments in different parts of Iran. A total of 130 tick specimens were collected from Hormozgan, Lorestan, and Guilan, Iran, within 2015 to 2017. Hyalomma asiaticum, Hyalomma dromedarii, Rhipicephalus sanguineus, and Rhipicephalus (Boophilus) species were identified in different geographical regions. An amplicon of 464-bp msp4 of Anaplasma was amplified using polymerase chain reaction in various tick species. Three sequences, including one Anaplasma marginale from R. (Boophilus) species and two Anaplasma ovis from Rhipicephalus sanguineus, were obtained after sequencing. It is concluded that bovine and ovine anaplasmosis agents are present in tick samples in Iran. The use of the gene families of six major surface proteins for the detection of various Anaplasma species is recommended.
本研究是基于伊朗不同地区主要表面蛋白4（msp4）基因片段测序，对蜱虫样本中的无形体属物种进行的首次分子检测。2015年至2017年期间，从伊朗霍尔木兹甘省、洛雷斯坦省和吉兰省共采集了130只蜱虫标本。在不同地理区域鉴定出了亚洲璃眼蜱、单峰驼璃眼蜱、血红扇头蜱和牛蜱属（微小牛蜱）物种。使用聚合酶链反应在各种蜱虫物种中扩增出了无形体464 bp的msp4扩增子。测序后获得了三个序列，包括一个来自牛蜱属（微小牛蜱）物种的边缘无形体和两个来自血红扇头蜱的绵羊无形体。得出的结论是，伊朗蜱虫样本中存在牛和羊的无形体病病原体。建议使用六种主要表面蛋白的基因家族来检测各种无形体属物种。
