Aubin Guillaume Ghislain, Ada Da Silva Grâce, Eishi Yoshinobu, Jacqueline Cédric, Altare Frédéric, Corvec Stéphane, Asehnoune Karim
EA3826, Laboratory of Clinical and Experimental Therapeutics of Infections, IRS 2, Nantes University, Nantes, France; Bacteriology and Hygiene Unit, CHU Nantes, France.
CRCINA, INSERM, Université d'Angers, Université de Nantes, Nantes, France.
Anaerobe. 2017 Dec;48:172-176. doi: 10.1016/j.anaerobe.2017.08.014. Epub 2017 Aug 30.
Cutibacterium (formerly Propionibacterium) acnes is involved in chronic/low-grade pathologies such as sarcoidosis or prosthetic joint infection (PJI). In these diseases, granulomatous structures are frequently observed. In this study, we induced a physiological granulomatous reaction in response to different well-characterized clinical C. acnes isolates in order to investigate the cellular process during granuloma formation. Three C. acnes isolates selected according to their origin (PJI, sarcoidosis and acne) were typed by MLST. All C. acnes isolates generated granulomatous structures in our experimental conditions. The bacterial burden was better controlled by granulomas induced by the sarcoidosis C. acnes isolate. The PJI C. acnes isolate, belonging to CC36, promoted the recruitment of CD8 lymphocytes inside the granuloma. In contrast, the acne and sarcoidosis C. acnes isolates, belonging to phylotypes IA/CC18 and IA/CC28, respectively, generated a higher number of granulomas and promoted the recruitment of CD4 lymphocytes inside the granuloma. Our results provide new evidence supporting the role of C. acnes in the development of sarcoidosis and new explanations concerning the mechanisms underlying PJI due to C. acnes.
痤疮丙酸杆菌（以前称为丙酸杆菌）与结节病或人工关节感染（PJI）等慢性/低度病理状况有关。在这些疾病中，经常观察到肉芽肿结构。在本研究中，我们针对不同特征明确的临床痤疮丙酸杆菌分离株诱导了生理性肉芽肿反应，以研究肉芽肿形成过程中的细胞过程。根据其来源（PJI、结节病和痤疮）选择的三株痤疮丙酸杆菌分离株通过多位点序列分型进行分型。在我们的实验条件下，所有痤疮丙酸杆菌分离株均产生了肉芽肿结构。结节病痤疮丙酸杆菌分离株诱导的肉芽肿对细菌负荷的控制效果更好。属于CC36的PJI痤疮丙酸杆菌分离株促进了CD8淋巴细胞在肉芽肿内的募集。相比之下，分别属于IA/CC18和IA/CC28菌型的痤疮和结节病痤疮丙酸杆菌分离株产生了更多的肉芽肿，并促进了CD4淋巴细胞在肉芽肿内的募集。我们的结果提供了新的证据，支持痤疮丙酸杆菌在结节病发展中的作用，并对痤疮丙酸杆菌引起PJI的潜在机制提供了新的解释。
