Hughson Bryon N, Anreiter Ina, Jackson Chornenki Nicholas L, Murphy Keith R, Ja William W, Huber Robert, Sokolowski Marla B
Department of Ecology and Evolutionary Biology, University of Toronto, 25 Willcocks Street, Toronto, Ontario M5S 3B2, Canada.
Department of Ecology and Evolutionary Biology, University of Toronto, 25 Willcocks Street, Toronto, Ontario M5S 3B2, Canada; Child and Brain Development Program, Canadian Institute for Advanced Research (CIFAR), 180 Dundas St. West, Suite 1400, Toronto, Ontario M5G 1Z8, Canada.
J Insect Physiol. 2018 Apr;106(Pt 1):20-29. doi: 10.1016/j.jinsphys.2017.08.011. Epub 2017 Aug 30.
We introduce a high-resolution adult foraging assay (AFA) that relates pre- and post-ingestive walking behavior to individual instances of food consumption. We explore the utility of the AFA by taking advantage of established rover and sitter strains known to differ in a number of feeding-related traits. The AFA allows us to effectively distinguish locomotor behavior in Fed and Food-Deprived (FD) rover and sitter foragers. We found that rovers exhibit more exploratory behavior into the center of an arena containing sucrose drops compared to sitters who hug the edges of the arena and exhibit thigmotaxic behavior. Rovers also discover and ingest more sucrose drops than sitters. Sitters become more exploratory with increasing durations of food deprivation and the number of ingestion events also increases progressively with prolonged fasting for both strains. AFA results are matched by strain differences in sucrose responsiveness, starvation resistance, and lipid levels, suggesting that under the same feeding condition, rovers are more motivated to forage than sitters. These findings demonstrate the AFA's ability to effectively discriminate movement and food ingestion patterns of different strains and feeding treatments.
我们介绍了一种高分辨率成年觅食测定法（AFA），该方法将摄食前后的行走行为与食物消耗的个体实例相关联。我们利用已知在许多与进食相关的性状上存在差异的既定漫游者和静坐者品系，探索AFA的效用。AFA使我们能够有效区分饱腹和食物剥夺（FD）的漫游者和静坐者觅食者的运动行为。我们发现，与紧贴竞技场边缘并表现出趋触性的静坐者相比，漫游者对含有蔗糖滴的竞技场中心表现出更多的探索行为。漫游者也比静坐者发现并摄取更多的蔗糖滴。随着食物剥夺时间的延长，静坐者变得更具探索性，并且两种品系的摄取事件数量也随着禁食时间的延长而逐渐增加。AFA的结果与蔗糖反应性、抗饥饿能力和脂质水平的品系差异相匹配，这表明在相同的进食条件下，漫游者比静坐者更有觅食动机。这些发现证明了AFA能够有效区分不同品系和进食处理的运动和食物摄取模式。
