黑腹果蝇幼虫的觅食路径长度协议
Foraging Path-length Protocol for Drosophila melanogaster Larvae.
作者信息
Anreiter Ina, Vasquez Oscar E, Allen Aaron M, Sokolowski Marla B
机构信息
Department of Ecology and Evolutionary Biology, University of Toronto.
Department of Cell and Systems Biology, University of Toronto.
出版信息
J Vis Exp. 2016 Apr 23(110):53980. doi: 10.3791/53980.
The Drosophila melanogaster larval path-length phenotype is an established measure used to study the genetic and environmental contributions to behavioral variation. The larval path-length assay was developed to measure individual differences in foraging behavior that were later linked to the foraging gene. Larval path-length is an easily scored trait that facilitates the collection of large sample sizes, at minimal cost, for genetic screens. Here we provide a detailed description of the current protocol for the larval path-length assay first used by Sokolowski. The protocol details how to reproducibly handle test animals, perform the behavioral assay and analyze the data. An example of how the assay can be used to measure behavioral plasticity in response to environmental change, by manipulating feeding environment prior to performing the assay, is also provided. Finally, appropriate test design as well as environmental factors that can modify larval path-length such as food quality, developmental age and day effects are discussed.
果蝇幼虫路径长度表型是一种既定的测量方法，用于研究行为变异的遗传和环境因素。幼虫路径长度测定法旨在测量觅食行为中的个体差异，这些差异后来与觅食基因相关联。幼虫路径长度是一种易于评分的性状，有助于以最低成本收集大量样本用于基因筛选。在此，我们详细描述了最初由索科洛夫斯基使用的幼虫路径长度测定法的当前方案。该方案详细说明了如何可重复地处理实验动物、进行行为测定以及分析数据。还提供了一个示例，说明如何通过在进行测定之前操纵饲养环境，来利用该测定法测量对环境变化的行为可塑性。最后，讨论了适当的测试设计以及可改变幼虫路径长度的环境因素，如食物质量、发育年龄和日效应。
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