Castell M, Gisbert M P, Queralt J, Castellote C
Allergol Immunopathol (Madr). 1987 Jan-Feb;15(1):29-32.
Total IgE and IgG serum levels were measured in rats treated with egg albumin (EA), Bordetella pertussis (Bp) and aluminium hydroxide gel (alum), and in rats administered with Bordetella pertussis or alum alone. Two ELISA micromethods have been developed to measure IgE and IgG changes. Immunoglobulin serum levels were evaluated 14 days after immunization. The highest IgE levels were obtained after sensitization with EA suspended in alum s.c. and administered with Bp i.p. The IgE production induced by Bp alone was significantly lower than that of the former. The IgE concentrations from alum treated group were the same as those obtained in the untreated animals. IgG serum levels remained unchanged in the different immunization procedures assayed.
在接受卵清蛋白（EA）、百日咳博德特氏菌（Bp）和氢氧化铝凝胶（明矾）处理的大鼠，以及仅给予百日咳博德特氏菌或明矾的大鼠中，测量了血清总IgE和IgG水平。已开发出两种ELISA微量法来测量IgE和IgG的变化。在免疫后14天评估免疫球蛋白血清水平。在用皮下注射于明矾中的EA致敏并腹腔注射Bp后，获得了最高的IgE水平。单独由Bp诱导的IgE产生明显低于前者。明矾处理组的IgE浓度与未处理动物的浓度相同。在所检测的不同免疫程序中，IgG血清水平保持不变。
