Wang Shuzhe, Pan Cuili, Ma Xiaojie, Yang Chaoyun, Tang Lin, Huang Jieping, Wei Xuefeng, Li Hui, Ma Yun
Key Laboratory of Ruminant Molecular and Cellular Breeding of Ningxia Hui Autonomous Region, School of Agriculture, Ningxia University, Yinchuan, China.
College of Life Sciences, Xinyang Normal University, Xinyang, China.
Front Genet. 2021 Dec 22;12:736441. doi: 10.3389/fgene.2021.736441. eCollection 2021.
The buffalo population is extensive in China, but its meat quality is relatively inferior. Therefore, improving meat quality should be one of the breeding goals. microRNAs (miRNAs) play an essential regulatory role in the post-transcriptional expression of genes. Some studies have reported their function regulating genes related to fat deposition and adipocyte differentiation in cattle, but there is limited reports in buffalo. We performed small RNA transcriptome sequencing of Xinyang buffalo adipose tissue between calves and adults in this study. As a result, 282 mature miRNAs were significantly differentially expressed, and co-expression analysis showed that 454 miRNAs were significantly associated with developmental stages. Target gene identification, GO (gene ontology) annotation, and KEGG analysis of miRNAs showed that miR-195, miR-192, and miR-24-3p could target key genes for lipogenesis and thus regulate adipose deposition and differentiation. Among them, miR-195 was significantly upregulated in adipose tissue and induced adipocytes of adult buffaloes, and its overexpression significantly inhibited lipid accumulation in primary adipocytes. Dual-luciferase reporter gene analysis showed that miR-195 reduced the expression of thyroid hormone response protein (THRSP) by targeting its 3' untranslated terminal region, suggesting that miR-195 may inhibit lipid accumulation in adipocytes by regulating . The results confirmed the reliability of predictive screening of miRNAs and provided theoretical support for buffalo fattening.
中国水牛群体数量庞大，但肉质相对较差。因此，改善肉质应成为育种目标之一。微小RNA（miRNA）在基因转录后表达中发挥着重要的调控作用。一些研究报道了它们在牛脂肪沉积和脂肪细胞分化相关基因调控中的功能，但在水牛方面的报道有限。在本研究中，我们对信阳水牛犊牛和成年牛的脂肪组织进行了小RNA转录组测序。结果显示，282个成熟miRNA存在显著差异表达，共表达分析表明454个miRNA与发育阶段显著相关。对miRNA的靶基因鉴定、基因本体（GO）注释和京都基因与基因组百科全书（KEGG）分析表明，miR-195、miR-192和miR-24-3p可靶向脂肪生成的关键基因，从而调控脂肪沉积和分化。其中，miR-195在成年水牛脂肪组织和诱导脂肪细胞中显著上调，其过表达显著抑制原代脂肪细胞中的脂质积累。双荧光素酶报告基因分析表明，miR-195通过靶向甲状腺激素反应蛋白（THRSP）的3'非翻译末端区域降低其表达，提示miR-195可能通过调控……抑制脂肪细胞中的脂质积累。研究结果证实了miRNA预测筛选的可靠性，为水牛育肥提供了理论支持。
