Morris R G, Hagan J J, Nadel L, Jensen J, Baudry M, Lynch G S
Behav Neural Biol. 1987 May;47(3):333-45. doi: 10.1016/s0163-1047(87)90448-1.
Rats were given continuous intraventricular infusion of saline or the thiol-proteinase inhibitor leupeptin, via subcutaneously implanted osmotic minipumps, while being trained on a spatial learning water task using spaced trials. Leupeptin caused overnight forgetting during training, but performance eventually reached asymptote in both groups. A retention test conducted 48 h later to assess spatial memory revealed no significant group differences, but did cause, in saline-treated rats only, a disruption of subsequent retraining back to the correct spatial location. The groups showed no differences in Cl-dependent [3H]glutamate receptor binding to hippocampal or entorhinal cortex membranes subsequent to training. In a second experiment, normal rats trained on the same task also showed no differences in Cl-dependent [3H]glutamate binding relative to rats exposed to the water task but given random spatial position training and handled controls. The results are discussed in relation to the hypothesis of Lynch and Baudry (Science (1984) 224, 1057-1063) that a calcium-dependent thiol proteinase is involved in memory formation through its ability to modify glutamate receptor distribution and dendritic spine shape.
通过皮下植入的渗透微型泵，给大鼠持续脑室内输注生理盐水或巯基蛋白酶抑制剂亮抑酶肽，同时使用间隔试验对其进行空间学习水迷宫任务训练。亮抑酶肽在训练期间导致大鼠一夜之间遗忘，但两组的表现最终都达到了渐近线。48小时后进行的一项用于评估空间记忆的记忆测试显示，两组之间没有显著差异，但仅在接受生理盐水处理的大鼠中，测试导致其后续重新训练回到正确空间位置时受到干扰。训练后，两组在氯离子依赖性[3H]谷氨酸受体与海马或内嗅皮质膜的结合方面没有差异。在第二个实验中，在相同任务上接受训练的正常大鼠，相对于接受水迷宫任务但进行随机空间位置训练的大鼠以及处理对照组，在氯离子依赖性[3H]谷氨酸结合方面也没有差异。根据林奇和博德里（《科学》（1984年）224卷，1057 - 1063页）的假设对结果进行了讨论，该假设认为钙依赖性巯基蛋白酶通过其改变谷氨酸受体分布和树突棘形状的能力参与记忆形成。
