Deyo R A, Conner R L
Department of Psychology, Bowling Green State University, Ohio 43403.
Behav Neural Biol. 1989 Sep;52(2):213-21. doi: 10.1016/s0163-1047(89)90327-0.
Adult male Long-Evans hooded rats were given bilateral microinjections of 18 mM leupeptin or saline through cannulae implanted with tips aimed at the frontal cortex or CA1 or CA3 hippocampal cell fields. Five minutes following injections animals were allowed to complete an eight-arm radial maze. The acquisition criterion required that the animal make 7 correct choices of the first 8, and 8 correct choices of the first 10, for five consecutive sessions. Leupeptin slowed acquisition of the eight-arm radial maze task when injected into the CA1 and CA3 hippocampal fields or the frontal cortex, but did not influence spontaneous activity. These results suggest that earlier reports of the amnestic effect of leupeptin when administered into the lateral cerebral ventricle may have been due to effects within the cortex and hippocampus. The present experiment represents the first attempt to identify behaviorally those brain areas in which leupeptin acts to alter learning.
成年雄性长 Evans 戴帽大鼠通过植入套管进行双侧微量注射，套管尖端对准额叶皮质、海马 CA1 区或 CA3 区细胞场，注射 18 mM 亮抑酶肽或生理盐水。注射后 5 分钟，让动物完成八臂放射状迷宫实验。获取标准要求动物在前 8 次中有 7 次正确选择，在前 10 次中有 8 次正确选择，且连续进行 5 次实验。当将亮抑酶肽注射到海马 CA1 区、CA3 区或额叶皮质时，会减缓八臂放射状迷宫任务的获取速度，但不影响自发活动。这些结果表明，早期关于将亮抑酶肽注入侧脑室时产生遗忘效应的报道可能是由于其对皮质和海马的影响。本实验首次尝试通过行为学方法确定亮抑酶肽发挥改变学习作用的脑区。
