Fabris C, Benini L, Del Favero G, Cavallini G, Basso D, Vantini I, Bonvicini P, Brocco G, Piccoli A, Tonon M
Enzyme. 1987;37(4):174-81. doi: 10.1159/000469259.
In order to investigate the role of circulating free trypsinogen and renal tubular dysfunction in affecting trypsin plasma-urine transfer, serum immunoreactive trypsin (IRT), its urinary output, IRT molecular size distribution, filtrable immunoreactive trypsin, gamma-glutamyltransferase and alpha-glucosidase outputs were studied in 6 control subjects, 9 patients with pancreatic cancer and 15 with chronic pancreatitis. The majority of immunoreactivity was always eluted at a molecular weight of about 24,000 and might therefore be considered as free trypsinogen. Variable amounts of IRT at higher molecular weights, possibly represented by trypsin-inhibitor complexes, were also detected. Increasing IRT levels were generally accounted for by free trypsinogen, regardless of the nature of the disease. Unlike serum free trypsinogen levels, renal tubular damage, evaluated by means of the excretion of two high-molecular weight urinary enzymes, seems to play a prominent role in explaining trypsin plasma-urine transfer.
为了研究循环游离胰蛋白酶原和肾小管功能障碍在影响胰蛋白酶血浆-尿液转运中的作用，我们对6名对照受试者、9名胰腺癌患者和15名慢性胰腺炎患者的血清免疫反应性胰蛋白酶（IRT）、其尿排出量、IRT分子大小分布、可滤过免疫反应性胰蛋白酶、γ-谷氨酰转移酶和α-葡萄糖苷酶排出量进行了研究。大多数免疫反应性总是在分子量约为24,000时洗脱，因此可能被视为游离胰蛋白酶原。还检测到了较高分子量的可变数量的IRT，可能由胰蛋白酶-抑制剂复合物代表。无论疾病性质如何，游离胰蛋白酶原通常是IRT水平升高的原因。与血清游离胰蛋白酶原水平不同，通过两种高分子量尿酶的排泄评估的肾小管损伤似乎在解释胰蛋白酶血浆-尿液转运中起重要作用。
