Fabris C, Benini L, Basso D, Del Favero G, Vantini I, Piccoli A, Cavallini G, Scuro L A, Naccarato R
Istituto di Medicina Interna (Cattedra di Malattie Apparato Digerente) Università degli Studi di Padova, Italy.
Pancreas. 1988;3(1):25-9. doi: 10.1097/00006676-198802000-00005.
In order to investigate the role of renal factors in affecting trypsinogen 1 metabolism and excretion in chronic pancreatic disease, serum immunoreactive trypsin (IRT), urinary IRT, gamma-glutamyltransferase (GGT), alpha-glucosidase (AGL) and RNase outputs and the molecular size distribution of serum and urine IRT were studied in 8 control subjects, 18 cases with pancreatic cancer, and 23 cases with chronic pancreatitis. Serum chromatography demonstrated that most immunoreactivity eluted as trypsinogen 1. Smaller amounts of immunoreactivity at higher molecular weights were also observed. Urine chromatography displayed both trypsinogen 1 and heavier molecular forms. An inverse linear correlation was noticed between creatinine clearance and serum trypsinogen 1 levels. Multiple regression analysis (urinary IRT output dependent and GGT, AGL, and RNase predictor variables) showed a significant linear correlation. RNase was found to be the most important parameter in explaining urinary IRT output. Mild variations in the glomerular function seem to be able to influence serum trypsinogen 1 levels. Urinary IRT excretion is principally explained by a disturbance in the tubular reabsorption of low molecular weight proteins, such as RNase.
为了研究肾脏因素在慢性胰腺疾病中对胰蛋白酶原1代谢和排泄的影响，我们对8名对照受试者、18例胰腺癌患者和23例慢性胰腺炎患者的血清免疫反应性胰蛋白酶（IRT）、尿IRT、γ-谷氨酰转移酶（GGT）、α-葡萄糖苷酶（AGL）和核糖核酸酶（RNase）输出量以及血清和尿液IRT的分子大小分布进行了研究。血清色谱分析表明，大多数免疫反应性以胰蛋白酶原1的形式洗脱。在较高分子量处也观察到少量的免疫反应性。尿液色谱显示了胰蛋白酶原1和分子量更大的形式。肌酐清除率与血清胰蛋白酶原1水平之间存在负线性相关。多元回归分析（以尿IRT输出量为因变量，GGT、AGL和RNase为预测变量）显示出显著的线性相关性。发现RNase是解释尿IRT输出量的最重要参数。肾小球功能的轻微变化似乎能够影响血清胰蛋白酶原1水平。尿IRT排泄主要是由低分子量蛋白质（如RNase）肾小管重吸收障碍所解释。
