School of Marine Science, Ningbo University, Ningbo, China.
Environ Sci Pollut Res Int. 2017 Oct;24(30):23704-23713. doi: 10.1007/s11356-017-0007-1. Epub 2017 Sep 1.
Vibrio bacteria live in both marine and freshwater habitats and are associated with aquatic animals. Vibrio vulnificus is a pathogenic bacterium that infects people and livestock. It is usually found in offshore waters or within fish and shellfish. This study presents a comparative proteomic analysis of the outer membrane protein (OMP) changes in V. vulnificus proteins after stimulation with sewage from sewage drains. Using two-dimensional electrophoresis followed by MALDI-TOF MS/MS, 32 protein spots with significant differences in abundance were identified and characterized. These identified proteins were found to be involved in various functional categories, including catalysis, transport, membrane proteins progresses, receptor activity, energy metabolism, cytokine activity, and protein metabolism. The mRNA expression levels of 12 differential proteins were further assessed by qRT-PCR. Seven genes including carboxypeptidase, hemoglobin receptor, succinate dehydrogenase iron-sulfur subunit, ATP synthase subunit alpha, thioredoxin, succinyl-CoA synthetase subunit, and alanine dehydrogenase were downregulated upon stimulation, whereas the protein expression levels HupA receptor, type I secretion outer membrane protein, glutamine synthetase, superoxide dismutase, OmpU, and VuuA were upregulated. H NMR spectra showed 18 dysregulated metabolites from V. vulnificus after the sewage stimulation and the pathogenicity was enhanced after that.
弧菌生活在海洋和淡水栖息地，并与水生动物有关。创伤弧菌是一种感染人和牲畜的病原菌。它通常存在于近岸水域或鱼类和贝类中。本研究对创伤弧菌蛋白在受到污水管污水刺激后的外膜蛋白（OMP）变化进行了比较蛋白质组学分析。通过二维电泳和 MALDI-TOF MS/MS，鉴定并鉴定了 32 个丰度有显著差异的蛋白点。这些鉴定出的蛋白质涉及多种功能类别，包括催化、运输、膜蛋白进展、受体活性、能量代谢、细胞因子活性和蛋白质代谢。通过 qRT-PCR 进一步评估了 12 种差异蛋白的 mRNA 表达水平。在刺激后，有 7 个基因（包括羧肽酶、血红蛋白受体、琥珀酸脱氢酶铁-硫亚基、ATP 合酶亚基 α、硫氧还蛋白、琥珀酰辅酶 A 合成酶亚基和丙氨酸脱氢酶）下调，而 HupA 受体、I 型分泌外膜蛋白、谷氨酰胺合成酶、超氧化物歧化酶、OmpU 和 VuuA 的蛋白表达水平上调。1H NMR 谱显示，创伤弧菌在受到污水刺激后有 18 种代谢物失调，此后其致病性增强。
