Zhang Dijun, He Shan, Ming Tinghong, Lu Chenyang, Zhou Jun, Su Xiurong
School of Marine Science, Ningbo University, 818 Fenghua Road, Ningbo, Zhejiang, 315211, People's Republic of China.
Arch Microbiol. 2017 Oct;199(8):1165-1173. doi: 10.1007/s00203-017-1383-0. Epub 2017 May 15.
Enterobacter cloacae is an opportunistic pathogen widely distributed in human and animal intestinal systems. The secretion of extended-spectrum β-lactamases (ESBLs) and cephalosporinase (AmpC) endows E. cloacae with strong drug resistance. In a previous study by our group, protein expression of E. cloacae under phoxim stress was measured by two-dimensional electrophoresis. Here, nuclear magnetic resonance was used to detect differences in E. cloacae metabonomics when under phoxim stress. We determined that there are 29 types of metabolites that differ between phoxim stress and normal culture conditions. Among these, 6 types of metabolites were upregulated in the phoxim stress group, and 23 types of metabolites were inhibited. Though enrichment analysis, seven pathways were identified by different expression levels of metabolites, which were involved in DNA and RNA synthesis, DNA damage repair, antioxidation and functions of the cell membrane and cell wall. The mechanism underlying how phoxim affects E. cloacae was determined by studying the results of both two-dimensional electrophoresis in our prior work and the analysis of E. cloacae metabonomic changes under phoxim stress.
阴沟肠杆菌是一种广泛分布于人和动物肠道系统中的机会致病菌。超广谱β-内酰胺酶（ESBLs）和头孢菌素酶（AmpC）的分泌赋予了阴沟肠杆菌强大的耐药性。在我们小组之前的一项研究中，通过二维电泳测量了辛硫磷胁迫下阴沟肠杆菌的蛋白质表达。在此，利用核磁共振检测阴沟肠杆菌在辛硫磷胁迫下代谢组学的差异。我们确定在辛硫磷胁迫和正常培养条件之间存在29种不同的代谢物。其中，辛硫磷胁迫组中有6种代谢物上调，23种代谢物受到抑制。通过富集分析，根据代谢物的不同表达水平确定了7条途径，这些途径涉及DNA和RNA合成、DNA损伤修复、抗氧化以及细胞膜和细胞壁的功能。通过研究我们之前工作中二维电泳的结果以及辛硫磷胁迫下阴沟肠杆菌代谢组学变化的分析，确定了辛硫磷影响阴沟肠杆菌的潜在机制。
