Section of Dental Pathology, Operative Dentistry and Endodontics, Department of Dentistry and Oral Health, HEALTH, Aarhus University, Vennelyst Boulevard 9, 8000, Aarhus C, Denmark.
Section of Oral Epidemiology and Dental Public Health, Department of Dentistry and Oral Health, HEALTH, Aarhus University, Vennelyst Boulevard 9, 8000, Aarhus C, Denmark.
Clin Oral Investig. 2018 Apr;22(3):1123-1129. doi: 10.1007/s00784-017-2195-y. Epub 2017 Sep 1.
The aim of this randomized, double-blind, crossover study was to measure fluoride in saliva and 7-day-old biofilm fluid and biofilm solids after rinsing three times per day for 3 weeks with 0, 1500, or 5000 ppm fluoride (NaF).
Following the 3-week wash-in/wash-out period, including 1 week of biofilm accumulation, saliva and biofilm samples were collected from 12 participants immediately before (background fluoride), and 10, 30, and 60 min after a single rinse. Biofilm samples were separated into fluid and solids, and samples were analyzed using a fluoride electrode (microanalysis).
The background fluoride concentration was statistically significantly higher in the 5000 compared to the 1500 ppm F rinse group in all three compartments (22.3 and 8.1 μM in saliva, 126.8 and 58.5 μM in biofilm fluid, and 10,940 and 4837 μmol/kg in biofilm solids). The 1-h fluoride accumulation for the 5000 ppm F rinse was higher than for the 1500 ppm F rinse in all three compartments, although not statistically significant for saliva and biofilm solids.
Regular exposure to 5000 ppm fluoride elevates background fluoride concentrations in saliva, biofilm fluid, and biofilm solids compared to 1500 ppm fluoride. Increasing the fluoride concentration almost 3.5 times (from 1500 to 5000 ppm) only elevates the background fluoride concentrations in saliva, biofilm fluid, and biofilm solids twofold.
Even though fluoride toothpaste may be diluted by saliva, the results of the present study indicate that use of 5000 ppm fluoride toothpaste might lead to improved caries control.
本随机、双盲、交叉研究的目的是测量每天用 0ppm、1500ppm 或 5000ppm 氟化物（NaF）漱口 3 次，共 3 周后，在唾液和 7 天龄生物膜液和生物膜固体中氟化物的含量。
在 3 周的冲洗/洗脱期后，包括 1 周的生物膜积累期，从 12 名参与者中收集了在单次冲洗前（背景氟化物）和冲洗后 10、30 和 60 分钟的唾液和生物膜样本。将生物膜样本分离为液体和固体，并用氟化物电极（微量分析）进行分析。
在所有三个隔室中，与 1500ppm F 冲洗组相比，5000ppm F 冲洗组的背景氟化物浓度在统计学上显著更高（唾液中的 22.3 和 8.1μM，生物膜液中的 126.8 和 58.5μM，以及生物膜固体中的 10940 和 4837μmol/kg）。在所有三个隔室中，5000ppm F 冲洗组的 1 小时氟化物积累高于 1500ppm F 冲洗组，但唾液和生物膜固体的差异没有统计学意义。
与 1500ppm 氟化物相比，5000ppm 氟化物的常规暴露会升高唾液、生物膜液和生物膜固体中的背景氟化物浓度。将氟化物浓度增加近 3.5 倍（从 1500ppm 增加到 5000ppm）只会使唾液、生物膜液和生物膜固体中的背景氟化物浓度增加两倍。
尽管氟化物牙膏可能会被唾液稀释，但本研究的结果表明，使用 5000ppm 氟化物牙膏可能会改善龋齿控制。
