Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, 5230 Odense M, Denmark.
Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, 5230 Odense M, Denmark.
Curr Opin Biotechnol. 2018 Feb;49:120-128. doi: 10.1016/j.copbio.2017.08.006. Epub 2017 Aug 30.
Small regulatory RNAs (sRNAs) are involved in post-transcriptional control of important cellular processes and contribute to the success of a pathogen. Here, we use studies primarily selected from Salmonella enterica and Listeria monocytogenes to illustrate the current status of sRNA biology in important foodborne pathogens. We discuss how the regulatory activities of sRNAs can be affected by base pairing RNAs known as 'sponge RNAs', or by RNA-binding proteins, such as the newly discovered sRNA chaperone ProQ. Furthermore, we highlight recent findings for sRNAs with regulatory roles during infection, some of which are present in multiple copies, designated 'sibling sRNAs'. Importantly, knowledge on sRNA-mediated regulation can be exploited for biotechnological applications, such as in generating gene knockdowns to promote desired traits.
小调控 RNA（sRNAs）参与重要细胞过程的转录后调控，并有助于病原体的成功。在这里，我们主要使用来自沙门氏菌和李斯特菌的研究来说明 sRNA 生物学在重要食源性病原体中的现状。我们讨论了 sRNA 的调节活性如何受到称为“海绵 RNA”的碱基配对 RNA 或 RNA 结合蛋白（如新发现的 sRNA 伴侣蛋白 ProQ）的影响。此外，我们强调了在感染过程中具有调节作用的 sRNA 的最新发现，其中一些具有多个副本，被指定为“姐妹 sRNA”。重要的是，基于 sRNA 介导的调控的知识可以被用于生物技术应用，例如产生基因敲低以促进所需特性。
