Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials, College of Chemistry and Chemical Engineering, Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules, Hubei University, Wuhan 430062, China.
Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials, College of Chemistry and Chemical Engineering, Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules, Hubei University, Wuhan 430062, China.
Mater Sci Eng C Mater Biol Appl. 2017 Nov 1;80:180-186. doi: 10.1016/j.msec.2017.03.227. Epub 2017 Mar 25.
In this study, we designed a novel molecularly imprinted polymer (MIP), Antimony Doped Tin Oxide (ATO)-silica composite sol, which was made using a sol-gel method. Then a sensitive and selective imprinted electrochemical sensor was constructed with the ATO-silica composite sol on a glassy carbon electrode modified by single-walled carbon nanotubes (SWNTs). The introduction of SWNTs increased the sensitivity of the MIP sensor. The surface morphology of the MIP and MIP/SWNTs were characterized by scanning electron microscopy (SEM), and the optimal conditions for detection were determined. The oxidative peak current increased linearly with the concentration of norepinephrine in the range of 9.99×10M to 1.50×10M, as detected by cyclic voltammetry (CV), the detection limit was 3.33×10M (S/N=3). In addition, the proposed electrochemical sensors were successfully applied to detect the norepinephrine concentration in human blood serum samples. The recoveries of the sensors varied from 99.67% to 104.17%, indicating that the sensor has potential for the determination of norepinephrine in clinical tests. Moreover, the imprinted electrochemical sensor was used to selectively detect norepinephrine. The analytical application was conducted successfully and yielded accurate and precise results.
在本研究中，我们设计了一种新型的分子印迹聚合物（MIP），即掺锑氧化锡（ATO）-二氧化硅复合溶胶，它是通过溶胶-凝胶法制备的。然后，我们在经过单壁碳纳米管（SWNTs）修饰的玻碳电极上构建了具有 ATO-二氧化硅复合溶胶的敏感和选择性印迹电化学传感器。SWNTs 的引入提高了 MIP 传感器的灵敏度。通过扫描电子显微镜（SEM）对 MIP 和 MIP/SWNTs 的表面形貌进行了表征，并确定了检测的最佳条件。通过循环伏安法（CV）检测，氧化峰电流在 9.99×10M 至 1.50×10M 的范围内与去甲肾上腺素的浓度呈线性关系，检测限为 3.33×10M（S/N=3）。此外，该电化学传感器还成功地应用于检测人血清样品中的去甲肾上腺素浓度。传感器的回收率在 99.67%至 104.17%之间变化，表明该传感器在临床测试中具有测定去甲肾上腺素的潜力。此外，印迹电化学传感器可用于选择性检测去甲肾上腺素。分析应用成功进行，结果准确且精确。
