Zhao Jian, Tu Keyao, Liu Yanlei, Qin Yulei, Wang Xiwei, Qi Lifeng, Shi Donglu
Key Laboratory of Rubber-Plastics Ministry of Education/Shandong Provincial Key Laboratory of Rubber-Plastics, Qingdao University of Science & Technology, No. 53 Zhengzhou Road, Qingdao 266042, China; The Materials Science and Engineering Program, Dept. of Mechanical and Materials Engineering, College of Engineering and Applied Science, University of Cincinnati, Cincinnati, OH 45221, USA.
Shanghai East Hospital, The Institute for Biomedical Engineering & Nano Science, Tongji University School of Medicine, Shanghai 200120, China.
Mater Sci Eng C Mater Biol Appl. 2017 Nov 1;80:88-92. doi: 10.1016/j.msec.2017.04.044. Epub 2017 Apr 29.
Dual surfaced dumbbell-like gold magnetic nanoparticles (Au-FeO) were synthesized for targeted aptamers delivery. Their unique biological properties were characterized as a smart photo-controlled drug carrier. DNA aptamers targeting vascular endothelial growth factor (VEGF) were assembled onto the surface of Au-FeO by electrostatic absorption. The binding capacity of the nanoparticles with VEGF aptamers was confirmed by gel electrophoresis. The targeted recognization of ovarian cancer cells by the aptamers-functionalized Au-FeO nanoparticles (Apt-Au-FeO NPs) was observed by confocal microscopy. Apt-Au-FeO was found to bind with SKOV-3 ovarian cancer cells specifically, leading to marked intracellular release of aptamers upon plasmon-resonant light (605nm) radiation, and to enhance the in vitro inhibition against tumor cell proliferation. The results show high potential of Apt-Au-FeOas a targeted cancer hyperthermia carrier by remote control with high spatial/temporal resolution.
合成了双表面哑铃状金磁性纳米颗粒（Au-FeO）用于靶向适体递送。其独特的生物学特性被表征为一种智能光控药物载体。通过静电吸附将靶向血管内皮生长因子（VEGF）的DNA适体组装到Au-FeO表面。通过凝胶电泳确认了纳米颗粒与VEGF适体的结合能力。通过共聚焦显微镜观察了适体功能化的Au-FeO纳米颗粒（Apt-Au-FeO NPs）对卵巢癌细胞的靶向识别。发现Apt-Au-FeO与SKOV-3卵巢癌细胞特异性结合，在等离子体共振光（605nm）辐射下导致适体在细胞内显著释放，并增强对肿瘤细胞增殖的体外抑制作用。结果表明，Apt-Au-FeO作为一种通过高空间/时间分辨率远程控制的靶向癌症热疗载体具有很高的潜力。
