Wang Siyuan, Cui Xinyun, Zhong Zixuan, Zhang Jingjing
State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Chemistry and Biomedicine Innovation Center (ChemBIC), Nanjing University, Nanjing 210023, China.
School of Life Sciences, Nanjing University, Nanjing 210023, China.
Biosensors (Basel). 2025 May 24;15(6):336. doi: 10.3390/bios15060336.
Photoactivatable aptamer sensing technology is widely used in various detection fields due to its precise spatiotemporal regulation ability, flexible material compatibility, and excellent detection performance. By introducing an optical response mechanism to regulate the efficient recognition of the target by the sensor, this strategy further broadens the regulation means of the aptamer. The application of photoactivated aptamer biosensors in point-of-care testing (POCT) can significantly improve the selectivity, sensitivity, and dynamic response ability of the POCT system. This review systematically explores the design principle and regulation mechanism of photoactivatable aptamers, with a focus on reviewing the application progress of them in the POCT platform. In addition, the existing challenges and future development trends are also discussed. It is expected that this biosensor based on photoactivatable aptamers will continue to drive POCT towards higher sensitivity, intelligence, and scene adaptability, providing innovative tools for precision medicine and environmental health monitoring.
光可激活适体传感技术因其精确的时空调控能力、灵活的材料兼容性和出色的检测性能，在各种检测领域得到广泛应用。通过引入光响应机制来调控传感器对目标的高效识别，该策略进一步拓宽了适体的调控手段。光激活适体生物传感器在即时检测（POCT）中的应用可显著提高POCT系统的选择性、灵敏度和动态响应能力。本文综述系统地探讨了光可激活适体的设计原理和调控机制，重点综述了它们在POCT平台上的应用进展。此外，还讨论了现有挑战和未来发展趋势。预计这种基于光可激活适体的生物传感器将继续推动POCT向更高灵敏度、智能化和场景适应性发展，为精准医学和环境卫生监测提供创新工具。
