Soper B D, Tepperman B L
J Physiol. 1986 Nov;380:329-40. doi: 10.1113/jphysiol.1986.sp016288.
Sialoadenectomized and sham-operated rats were given salivary secretory stimulants 30 min prior to intragastric instillation of a bile salt solution (5 mM-sodium taurocholate in 100 mM-HCl). Administration of the alpha-agonist phenylephrine (0.15-15 mg/kg) resulted in a dose-dependent reduction in the loss of H+ and the intraluminal appearance of Na+ and K+ associated with bile-salt-induced damage to the stomach in the sham-sialoadenectomized rat. The effect was not apparent if the salivary glands had been previously excised. Adrenaline (0.8-4.0 mg/kg) and noradrenaline (0.8-4.0 mg/kg) were less effective in reducing the degree of mucosal damage in sham-sialoadenectomized rats and were not effective in sialoadenectomized rats. Administration of secretory stimulant doses of isoprenaline (5 mg/kg), pilocarpine (2 mg/kg) and substance P (25 mg/kg) either had no significant effect or exacerbated the net transmucosal fluxes of H+, Na+ and K+ associated with bile salt damage to the gastric mucosa. The protective action of phenylephrine in sham-sialoadenectomized rats was reversed by prior treatment with the alpha-antagonist, phentolamine (2 mg/kg). The effect of phentolamine was dose dependent. Vagotomy abolished the protective influence of phenylephrine in sham-sialoadenectomized rats but did not influence the response to other salivary secretory stimulants consistently. These data suggest that stimulation of alpha-adrenergic receptors in rat salivary tissue is associated with an amelioration of the increase in gastric mucosal permeability to H+, Na+ and K+ in response to an intraluminal bile salt solution. The apparent protective influence of alpha-adrenergic receptor activation in sham-sialoadenectomized rats is mediated in part by the vagus nerve.
在向唾液腺切除和假手术的大鼠胃内灌注胆盐溶液（100 mM - HCl 中的 5 mM 牛磺胆酸钠）前 30 分钟，给予唾液分泌刺激剂。给予α-激动剂去氧肾上腺素（0.15 - 15 mg/kg）导致假唾液腺切除大鼠中与胆盐诱导的胃损伤相关的 H⁺丢失以及 Na⁺和 K⁺腔内出现呈剂量依赖性减少。如果唾液腺先前已被切除，则该效应不明显。肾上腺素（0.8 - 4.0 mg/kg）和去甲肾上腺素（0.8 - 4.0 mg/kg）在减轻假唾液腺切除大鼠的黏膜损伤程度方面效果较差，并且在唾液腺切除大鼠中无效。给予分泌刺激剂量的异丙肾上腺素（5 mg/kg）、毛果芸香碱（2 mg/kg）和 P 物质（25 mg/kg）要么没有显著影响，要么加剧了与胆盐对胃黏膜损伤相关的 H⁺、Na⁺和 K⁺的净跨黏膜通量。在假唾液腺切除大鼠中，预先用α-拮抗剂酚妥拉明（2 mg/kg）处理可逆转去氧肾上腺素的保护作用。酚妥拉明的作用是剂量依赖性的。迷走神经切断术消除了去氧肾上腺素在假唾液腺切除大鼠中的保护作用，但并未始终一致地影响对其他唾液分泌刺激剂的反应。这些数据表明，刺激大鼠唾液组织中的α-肾上腺素能受体与减轻胃黏膜对 H⁺、Na⁺和 K⁺的通透性增加有关，该通透性增加是对腔内胆盐溶液的反应。在假唾液腺切除大鼠中，α-肾上腺素能受体激活的明显保护作用部分由迷走神经介导。
