Tepperman B L, Soper B D
Dig Dis Sci. 1984 Jan;29(1):56-64. doi: 10.1007/BF01296862.
Extirpation of the major salivary glands in rats has been shown to exacerbate the ionic fluxes associated with pharmacologically-induced damage to the gastric mucosal barrier. In the present study we have investigated the effect of the administration of a crude extract of submandibular-sublingual salivary glands on gastric secretion and gastric mucosal barrier integrity in the rat. The extract was administered in a dose (4 mg protein) which would reduce established acid secretion by 30%. Chronic parenteral (intraperitoneal) administration of salivary gland extract but not phosphate buffer resulted in a reduction in the luminal appearance of Na+ and K+ associated with bile salt-induced damage (5 mM sodium taurocholate in 150 mM HCl) to the gastric mucosa of sialoadenectomized animals. Oral administration of the extract depressed its protective action. Boiling the extract prior to parenteral administration also abolished its ability to protect the mucosa. Acute administration of the extract by intraperitoneal injection only 30 min prior to bile salt treatment was also ineffective. These results suggest that chronic parenteral but not enteral treatment with a crude salivary gland extract aids in the maintenance of the gastric mucosal barrier in sialoadenectomized rats. The inability of acute administration of the extract to reduce the net fluxes of Na+ and K+ suggests that its mode of action is not entirely dependent upon an inhibition of gastric acid secretion.
已表明切除大鼠的主要唾液腺会加剧与药理学诱导的胃黏膜屏障损伤相关的离子通量。在本研究中，我们研究了给予大鼠下颌下-舌下唾液腺粗提物对胃分泌和胃黏膜屏障完整性的影响。提取物以一定剂量（4毫克蛋白质）给药，该剂量可使已有的胃酸分泌减少30%。唾液腺提取物而非磷酸盐缓冲液的慢性肠外（腹腔内）给药导致与胆汁盐诱导的损伤（在150毫摩尔盐酸中加入5毫摩尔牛磺胆酸钠）相关的唾液腺切除动物胃黏膜中Na+和K+的管腔外观减少。提取物的口服给药降低了其保护作用。在肠外给药前将提取物煮沸也消除了其保护黏膜的能力。仅在胆汁盐处理前30分钟通过腹腔注射急性给予提取物也无效。这些结果表明，唾液腺粗提物的慢性肠外而非肠内治疗有助于维持唾液腺切除大鼠的胃黏膜屏障。提取物急性给药无法减少Na+和K+的净通量表明其作用方式并不完全依赖于对胃酸分泌的抑制。
