Graduate School of Microbiology, Department of Biology, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University, Darmaga Campus, Bogor, West Java, 16880, Indonesia.
Department of Biology, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University, Darmaga Campus, Bogor, West Java, 16880, Indonesia.
Mycorrhiza. 2018 Jan;28(1):85-92. doi: 10.1007/s00572-017-0798-4. Epub 2017 Sep 2.
This research aimed to study the ectomycorrhiza formed by basidiospores attached to the outer surface of Shorea leprosula (Dipterocarpaceae) seed collected from a lowland tropical dipterocarp forest. Two groups of seeds were collected: control seeds collected from plastic net hanging 2 m above the ground and forest floor seeds collected on the forest floor. Before planting, 15 seeds from each group were observed for basidiospores attached to the seed. Ten of the 3-week-old S. leprosula seedlings of each group were individually grown in 1.5 kg of sterile zeolite for 8 months in a greenhouse. Pots were fertilized with MMN solution containing half the strength of phosphate. Fungal identity, ectomycorrhizal root tip colonization and anatomy, plant growth, and phosphate uptake were measured. The control seeds did not have basidiospores attached, whereas the forest floor seeds had 2 × 10 basidiospores of Tomentella. Bioassay test results indicate that the seedlings from the control seeds did not form ectomycorrhiza, whereas those seedlings from the forest floor seeds formed 3 morphotypes of ectomycorrhizae. Based on ITS1, 5.8S, and ITS2 rDNA region analyses, the 3 morphotypes belonged to Tomentella sp. HBT2, Tomentella sp. HBT4, and Scleroderma sp. HBS3. Root colonization percentage was above 70% for all three morphotypes. Root colonization in general increased plant growth and phosphate uptake. This is the first report of Tomentella basidiospores attached on the seed surface as a functional inoculum and of Tomentella ectomycorrhiza from dipterocarps lowland tropical forest.
本研究旨在研究从低地热带龙脑香林采集的波罗蜜（龙脑香科）种子外表面附着的担子孢子形成的外生菌根。收集了两组种子：一组是从离地 2 米高的塑料网中收集的对照种子，另一组是从林地上收集的对照种子。在种植前，观察每组的 15 颗种子是否附着有担子孢子。每组的 3 周龄幼苗各 10 株分别在温室中无菌沸石 1.5 公斤中单独种植 8 个月。盆中用含有磷酸盐一半浓度的 MMN 溶液施肥。测量了真菌种类、外生菌根根尖定殖和解剖结构、植物生长和磷酸盐吸收。对照种子没有附着担子孢子，而林地板种子有 2×10 个 Tomentella 的担子孢子。生物测定试验结果表明，对照种子的幼苗没有形成外生菌根，而林地板种子的幼苗形成了 3 种外生菌根形态。基于 ITS1、5.8S 和 ITS2 rDNA 区分析，这 3 种形态属于 Tomentella sp. HBT2、Tomentella sp. HBT4 和 Scleroderma sp. HBS3。所有三种形态的根定殖率均超过 70%。根定殖普遍增加了植物的生长和磷酸盐的吸收。这是首次报道在波罗蜜种子表面附着担子孢子作为功能接种体，以及报道低地热带龙脑香林的外生菌根来自龙脑香科植物。
