Yuzuncu Yil University, Medical Faculty, Department of Internal Medicine, Van, Turkey.
Yuzuncu Yil University, Medical Faculty, Department of Otorhinolaryngology, Van, Turkey.
Clin Chim Acta. 2017 Oct;473:186-190. doi: 10.1016/j.cca.2017.08.039. Epub 2017 Sep 1.
The relationship between increased serum enzyme activity of prolidase and increased rate of collagen turnover in the arterial wall has been asserted in previous studies. Collagen reflects much of the strength to the connective tissue involved in the arterial wall. Atherosclerosis is very common vessel disease and oxidative stress plays a pivotal role in the etiopathogenesis. Our objective was to examine the serum enzyme activity of prolidase and its possible relationships with oxidative stress parameters in obese subjects.
Our present study was conducted 27 obese subjects and 26 age-matched healthy control subjects. The serum enzyme activity of prolidase in all study population was evaluated spectrophotometrically. Oxidative stress levels in obese subjects were analyzed with total antioxidant capacity (TAC) and total oxidant status (TOS) as well as oxidative stress index (OSI).
Obese subjects have higher serum TOS and OSI indicators as well as prolidase activity than those in control subjects (for all; p<0.001). Moreover, obese subjects have lower levels of TAC than in those in healthy subjects (p<0.001). In the Pearson's correlation analysis, enzyme activity of prolidase was positively related with TOS (p<0.001, r=0.529) and OSI (p<0.001, r=0.519) as well as BMI (p<0.001, r=0.692) and inversely related with TAC (p<0.05, r=-0.405) in obese subjects.
Increased serum prolidase activity and decreased antioxidant levels are likely to be a results of increased of oxidative stress levels in obese subjects. The significantly correlation between increased oxidative stress and increased prolidase activity may play a pivotal role in etiopathogenesis of atherosclerotic cardiovascular diseases in obese subjects.
先前的研究已经证实，脯氨酸酶血清酶活性的增加与动脉壁中胶原蛋白周转率的增加之间存在关系。胶原蛋白反映了动脉壁中参与的结缔组织的大部分强度。动脉粥样硬化是一种非常常见的血管疾病，氧化应激在发病机制中起着关键作用。我们的目的是检查肥胖个体中脯氨酸酶的血清酶活性及其与氧化应激参数的可能关系。
本研究共纳入 27 名肥胖患者和 26 名年龄匹配的健康对照者。所有研究对象的脯氨酸酶血清酶活性均采用分光光度法进行评估。通过总抗氧化能力（TAC）、总氧化状态（TOS）和氧化应激指数（OSI）分析肥胖患者的氧化应激水平。
与对照组相比，肥胖组的血清 TOS 和 OSI 指标以及脯氨酸酶活性更高（所有 P<0.001）。此外，肥胖组的 TAC 水平低于健康对照组（P<0.001）。在 Pearson 相关分析中，脯氨酸酶的酶活性与 TOS（P<0.001，r=0.529）和 OSI（P<0.001，r=0.519）呈正相关，与 BMI（P<0.001，r=0.692）呈正相关，与 TAC（P<0.05，r=-0.405）呈负相关。
肥胖患者血清脯氨酸酶活性增加和抗氧化水平降低可能是氧化应激水平升高的结果。氧化应激增加与脯氨酸酶活性增加之间的显著相关性可能在肥胖患者动脉粥样硬化性心血管疾病的发病机制中起关键作用。
