Igarashi Hirotaka, Ohno Koichi, Matsuki Naoaki, Fujiwara-Igarashi Aki, Kanemoto Hideyuki, Fukushima Kenjiro, Uchida Kazuyuki, Tsujimoto Hajime
Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
Department of Radiation Biology, School of Veterinary Medicine, Rakuno Gakuen University, 582 Bunkyodai-Midorimachi, Ebetsu, Hokkaido 069-8501, Japan.
J Vet Med Sci. 2017 Oct 20;79(10):1727-1734. doi: 10.1292/jvms.17-0165. Epub 2017 Sep 4.
Short chain fatty acids (SCFAs) play an important role in the maintenance of colonic homeostasis, and their depletion has been reported in various gastrointestinal disorders. Inflammatory colorectal polyps (ICRPs) are a recently recognized disease specific to miniature dachshunds (MDs), and fecal dysbiosis with a reduction of SCFA-producing bacteria has been reported with this disease. Therefore, this study was performed based on the hypothesis that a reduced SCFA concentration associates with the development of ICRPs. We recruited 11 ICRP-affected MDs and 25 control MDs. Their fecal SCFA concentrations and bacterial proportions were quantified using high performance liquid chromatography and quantitative real-time PCR, respectively. The feces of ICRP-affected MDs contained lower amounts of propionic acid and lower proportions of Bifidobacterium than the feces of control MDs. Furthermore, fecal proportions of Bifidobacterium, Firmicutes and Lactobacillus exhibited significant positive correlations with fecal concentrations of total SCFAs and/or propionic acid; fecal Escherichia coli proportions correlated negatively with fecal concentrations of total SCFAs, as well as acetic, propionic and butyric acid. This result indicates an association between fecal dysbiosis and fecal SCFA concentrations; these phenomena may contribute to ICRP pathogenesis in MDs. Potential therapeutic targeting of the reduced propionic acid concentration using probiotics, prebiotics or SCFA enemas merits further study.
短链脂肪酸（SCFAs）在维持结肠内环境稳定中发挥着重要作用，并且在各种胃肠道疾病中均有报道其含量减少。炎症性结肠息肉（ICRPs）是最近才被认识到的一种迷你腊肠犬（MDs）特有的疾病，据报道，这种疾病存在粪便微生物群失调，产生SCFA的细菌数量减少。因此，本研究基于SCFA浓度降低与ICRPs发生有关的假设进行。我们招募了11只受ICRPs影响的MDs和25只对照MDs。分别使用高效液相色谱法和定量实时PCR对它们的粪便SCFA浓度和细菌比例进行了定量分析。与对照MDs的粪便相比，受ICRPs影响的MDs的粪便中丙酸含量较低，双歧杆菌比例也较低。此外，双歧杆菌、厚壁菌门和乳酸杆菌在粪便中的比例与总SCFAs和/或丙酸的粪便浓度呈显著正相关；粪便中大肠杆菌的比例与总SCFAs以及乙酸、丙酸和丁酸的粪便浓度呈负相关。这一结果表明粪便微生物群失调与粪便SCFA浓度之间存在关联；这些现象可能导致MDs中ICRPs的发病机制。使用益生菌、益生元或SCFA灌肠剂针对降低的丙酸浓度进行潜在治疗值得进一步研究。
