Division of Rheumatology, Department of Internal Medicine, Soonchunhyang University Seoul Hospital, Seoul, 04401, Republic of Korea.
Probiotics Microbiome Convergence Center, Soonchunhyang University, Asan, 31538, Republic of Korea.
J Microbiol. 2022 Dec;60(12):1178-1190. doi: 10.1007/s12275-022-2416-1. Epub 2022 Nov 24.
Microbiome research has been on the rise recently for a more in-depth understanding of gout. Meanwhile, there is a need to understand the gut microbiome related to uric acid-lowering drug resistance. In this study, 16S rRNA gene-based microbiota analysis was performed for a total of 65 stool samples from 17 healthy controls and 48 febuxostat-treated gout patients (including 28 controlled subjects with decreased uric acid levels and 20 uncontrolled subjects with non-reduced uric acid levels). Alpha diversity of bacterial community decreased in the healthy control, controlled, and uncontrolled groups. In the case of beta diversity, the bacterial community was significantly different among groups (healthy control, controlled, and uncontrolled groups). Taxonomic biomarker analysis revealed the increased population of g-Bifidobacterium in healthy controls and g-Prevotella in uncontrolled patients. PCR further confirmed this result at the species level. Additionally, functional metagenomics predictions led to the exploration of various functional biomarkers, including purine metabolism. The results of this study can serve as a basis for developing potential new strategies for diagnosing and treating gout from microbiome prospects.
近年来，为了更深入地了解痛风，微生物组研究呈上升趋势。同时，需要了解与降低尿酸药物耐药性相关的肠道微生物组。在这项研究中，对 17 名健康对照者和 48 名非布司他治疗的痛风患者（包括 28 名尿酸水平降低的对照者和 20 名尿酸水平未降低的对照者）的 65 份粪便样本进行了基于 16S rRNA 基因的微生物组分析。在健康对照组、对照组和未对照组中，细菌群落的 alpha 多样性均降低。在 beta 多样性方面，各组之间的细菌群落存在显著差异（健康对照组、对照组和未对照组）。分类生物标志物分析显示，健康对照组中 g-Bifidobacterium 的种群增加，而未对照组中 g-Prevotella 的种群增加。PCR 进一步在物种水平上证实了这一结果。此外，功能宏基因组学预测导致了各种功能生物标志物的探索，包括嘌呤代谢。本研究结果可为从微生物组角度开发痛风的潜在新诊断和治疗策略提供依据。
