Parab Pankaj, Khandeparker Rakhee, Amberkar Ujwala, Khodse Vishwas
Biological Oceanography Department, National Institute of Oceanography, Donapaula, Goa 403004 India.
3 Biotech. 2017 Oct;7(5):296. doi: 10.1007/s13205-017-0921-4. Epub 2017 Aug 31.
Enzymatic hydrolysis of seaweed biomass was studied using xylanase produced from marine bacteria sp. strain BT21 through solid-state fermentation of wheat bran. Three types of seaweeds, , and , were selected as representatives of red, brown, and green seaweeds, respectively. Seaweed biomass was pretreated with hot water. The efficiency of pretreated biomass to release reducing sugar by the action of xylanase as well as the type of monosaccharide released during enzyme saccharification of seaweed biomass was studied. It was seen that pretreated biomass of seaweed , and , at 121 °C for 45 min, followed by incubation with 50 IU xylanase released reducing sugars of 233 ± 5.3, 100 ± 6.1 and 73.3 ± 4.1 µg/mg of seaweed biomass, respectively. Gas chromatography analysis illustrated the release of xylose, glucose, and mannose during the treatment process. Hot water pre-treatment process enhanced enzymatic conversion of biomass into sugars. This study revealed the important role of xylanase in saccharification of seaweed, a promising feedstock for third-generation bioethanol production.
利用海洋细菌sp.菌株BT21通过麸皮固态发酵产生的木聚糖酶，对海藻生物质的酶解进行了研究。分别选取三种海藻，即紫菜、海带和浒苔，作为红藻、褐藻和绿藻的代表。海藻生物质用热水进行预处理。研究了预处理后的生物质在木聚糖酶作用下释放还原糖的效率，以及海藻生物质酶糖化过程中释放的单糖类型。结果表明，预处理后的紫菜、海带和浒苔生物质在121℃下处理45分钟，然后与50 IU木聚糖酶孵育，分别释放出233±5.3、100±6.1和73.3±4.1 μg/mg海藻生物质的还原糖。气相色谱分析表明，处理过程中释放出木糖、葡萄糖和甘露糖。热水预处理过程提高了生物质向糖的酶促转化。本研究揭示了木聚糖酶在海藻糖化中的重要作用，海藻是第三代生物乙醇生产的一种有前景的原料。
