Key Laboratory of Mountain Ecological Restoration and Bioresource Utilization, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, PR China.
J Pharm Biomed Anal. 2011 Dec 15;56(5):1089-93. doi: 10.1016/j.jpba.2011.07.034. Epub 2011 Jul 30.
A validated HPLC-DAD-ESI-MS method has been developed to simultaneously quantify 12 bioactive compounds in the seeds of Notopterygium incisum Ting ex H.T. Chang and Notopterygium franchetii H. de Boiss whose rhizomes and roots are widely used as traditional Chinese medicine. This method was validated to be sensitive, precise and accurate and was applied to evaluate the difference in the chemical profiles and contents of these analytes in 37 batches of N. incisum and 31 batches of N. franchetii samples collected from different locations. Principal component analysis showed that the two species were separated into two groups obviously. This study established a validated method for identification of the authenticity of the seeds of N. incisum and N. franchetii and supplied effective guidance for artificial cultivation.
建立了一种同时定量测定独活和羌活种子中 12 种生物活性成分的 HPLC-DAD-ESI-MS 方法，独活和羌活的根茎和根广泛用作中药。该方法经验证具有灵敏度高、精密度和准确度好的特点，并应用于评价 37 批独活和 31 批羌活样品中这些分析物的化学特征和含量的差异。主成分分析表明，这两个种明显分为两组。本研究建立了一种鉴定独活和羌活种子真实性的有效方法，为人工栽培提供了有效指导。
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