Artimani T, Karimi J, Mehdizadeh M, Yavangi M, Khanlarzadeh E, Ghorbani M, Asadi S, Kheiripour N
a Endometrium and Endometriosis Research Center , Hamadan University of Medical Sciences , Hamadan , Iran.
b Department of Clinical Biochemistry , Hamadan University of Medical Sciences , Hamadan , Iran.
Gynecol Endocrinol. 2018 Feb;34(2):148-152. doi: 10.1080/09513590.2017.1371691. Epub 2017 Sep 4.
Chronic low-grade inflammation has been suggested as a key contributor of the pathogenesis and development of polycystic ovary syndrome (PCOS). To investigate the association between oxidative stress status and inflammatory cytokines in follicular fluid of 21 PCOS women compared to 21 women with normal ovarian function who underwent intra-cytoplasmic sperm injection. Concentration of IL-6, IL-8, IL-10, and TNF-α was measured using sandwich ELISA. Oxidative stress was examined by measuring total oxidant status (TOS), malondialdehyde (MDA), total antioxidant capacity (TAC), and thiol groups. PCOS women had an elevated concentration of MDA and TOS compared to controls. Levels of TAC and thiol groups were lower in PCOS compared to controls. PCOS patients had a higher concentration of IL-6, IL-8, and TNF-α compared to controls. Concentration of IL-10 was lower in PCOS compared to controls. Significant correlations were found between MDA and TOS concentration with TNF-α and between IL-6 and MDA, IL-8 and TAC, IL-10 and TOS levels and also between IL-10 and TAC levels. TAC and thiol groups were negatively correlated with TNF-α. Increased oxidative stress in PCOS is associated with inflammation which is closely linked. Inflammation can induce production of inflammatory cytokines in this syndrome and directly stimulates excess ovarian androgen production.
慢性低度炎症被认为是多囊卵巢综合征（PCOS）发病机制和发展的关键因素。为了研究21例PCOS女性与21例接受胞浆内单精子注射且卵巢功能正常的女性卵泡液中氧化应激状态与炎性细胞因子之间的关联。采用夹心ELISA法测定IL-6、IL-8、IL-10和TNF-α的浓度。通过测量总氧化剂状态（TOS）、丙二醛（MDA）、总抗氧化能力（TAC）和巯基来检测氧化应激。与对照组相比，PCOS女性的MDA和TOS浓度升高。与对照组相比，PCOS患者的TAC和巯基水平较低。与对照组相比，PCOS患者的IL-6、IL-8和TNF-α浓度较高。与对照组相比，PCOS患者的IL-10浓度较低。发现MDA和TOS浓度与TNF-α之间以及IL-6与MDA、IL-8与TAC、IL-10与TOS水平之间存在显著相关性，并且IL-10与TAC水平之间也存在显著相关性。TAC和巯基与TNF-α呈负相关。PCOS中氧化应激增加与炎症相关，二者紧密相连。炎症可诱导该综合征中炎性细胞因子的产生，并直接刺激卵巢雄激素过度分泌。
