Huang Zhaobin, Lai Qiliang, Shao Zongze
Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, PR China.
State Key Laboratory Breeding Base of Marine Genetic Resources, Xiamen 361005, PR China.
Int J Syst Evol Microbiol. 2017 Sep;67(9):3532-3534. doi: 10.1099/ijsem.0.002160. Epub 2017 Sep 4.
Pleomorphobacterium xiamenense CLWT was compared with Oceanicella actignis PRQ-67T to examine the taxonomic relationship between the two organisms. The 16S rRNA gene sequence comparison showed that the two strains had 99.9 % sequence similarity. Phylogenetic analysis showed the two strains formed an independent tight cluster, distinctly branching from the closely related species in the family Rhodobacteraceae. Whole genomic comparison between the two strains revealed a digital DNA-DNA hybridization estimate of 88.4 % and average nucleotide identity of 98.8 %, strongly supporting that the two strains represented a single species. In addition, neither strain displayed any striking difference in biochemical characteristics, fatty acid composition, and polar lipid profile. According to priority, Pleomorphobacterium xiamenense is reclassified as a later heterotypic synonym of Oceanicella actignis based on the phylogenetic relationship, whole genomic comparison, fatty acid composition and polar lipid profile, and other phenotypic and biochemical properties.
将厦门多形杆菌CLWT与活动海洋杆菌PRQ-67T进行比较，以研究这两种微生物之间的分类关系。16S rRNA基因序列比较显示，这两个菌株的序列相似性为99.9%。系统发育分析表明，这两个菌株形成了一个独立的紧密聚类，明显不同于红杆菌科中密切相关的物种。两个菌株之间的全基因组比较显示，数字DNA-DNA杂交估计值为88.4%，平均核苷酸同一性为98.8%，有力地支持这两个菌株代表一个单一物种。此外，两个菌株在生化特性、脂肪酸组成和极性脂质谱方面均未显示出任何显著差异。根据优先权，基于系统发育关系、全基因组比较、脂肪酸组成和极性脂质谱以及其他表型和生化特性，厦门多形杆菌被重新分类为活动海洋杆菌的晚出异名。
