Huang Zhaobin, Lai Qiliang, Shao Zongze
State Key Laboratory Breeding Base of Marine Genetic Resources, Xiamen 361005, PR China.
Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, PR China.
Int J Syst Evol Microbiol. 2017 Jun;67(6):2043-2045. doi: 10.1099/ijsem.0.001982.
Salipiger nanhaiensis ZH114T was compared with Thiobacimonas profunda JLT2016T to clarify their taxonomic relationship. The results of the 16S rRNA gene sequence comparison indicated that the two strains had 99.7 % sequence similarity. Results of phylogenetic analysis indicated that the two strains formed a tight cluster, distinctly branching from Salipiger mucosus A3T and other representatives in the family Rhodobacteraceae. Whole genomic comparison between the two strains yielded a digital DDH estimate of 97.3 % and ANI value of 99.7 %, strongly supporting the hypothesis that the two strains represented a single species. In addition, the two strains displayed no striking differences in physiological and biochemical properties, fatty acid composition or polar lipids profile. Due to priority, Salipiger nanhaiensis is reclassified as a later heterotypic synonym of Thiobacimonas profunda based on the phylogenetic relationship inferred from the 16S rRNA gene sequence, whole genomic comparison, fatty acid composition and polar lipid profile and other phenotypic and biochemical properties.
将南海盐栖菌ZH114T与深海硫杆菌JLT2016T进行比较，以阐明它们的分类关系。16S rRNA基因序列比较结果表明，这两株菌的序列相似性为99.7%。系统发育分析结果表明，这两株菌形成了一个紧密的聚类，明显与黏液盐栖菌A3T及红杆菌科的其他代表菌株分支。两株菌的全基因组比较得出的数字DNA-DNA杂交估计值为97.3%，平均核苷酸一致性值为99.7%，有力支持了这两株菌代表单一物种的假设。此外，这两株菌在生理生化特性、脂肪酸组成或极性脂质谱方面没有显著差异。基于从16S rRNA基因序列、全基因组比较、脂肪酸组成和极性脂质谱以及其他表型和生化特性推断出的系统发育关系，由于优先权问题，南海盐栖菌被重新分类为深海硫杆菌的晚出异名。
