Liu Yang, Lai Qiliang, Xu Xue-Wei, Wu Yue-Hong, Cheng Hong, Zhang Xiao-Hua, Wang Long, Shao Zongze
State Key Laboratory Breeding Base of Marine Genetic Resources; Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, SOA; Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources; Key Laboratory of Marine Genetic Resources of Fujian Province, Xiamen 361005, PR China.
Laboratory of Marine Ecosystem and Biogeochemistry, Second Institute of Oceanography, State Oceanic Administration, Hangzhou 310012, PR China.
Int J Syst Evol Microbiol. 2017 Aug;67(8):3095-3098. doi: 10.1099/ijsem.0.002097. Epub 2017 Aug 22.
A polyphasic taxonomic study was undertaken to clarify the exact position of type strain DY6-4T of Xuhuaishuia manganoxidans. A combination of physiological properties of X. manganoxidans DY6-4T was consistent with those of type strain 22DY15T of Brevirhabdus pacifica. The 16S rRNA gene sequence analyses indicated that X. manganoxidans DY6-4T and B. pacifica 22DY15T shared 100 % similarity and formed a monophyletic group. The close relationship between the two strains was underpinned by the results of chemotaxonomic characteristics, including the fatty acids, quinone and polar lipids. The digital DNA-DNA hybridization and average nucleotide identity values between the two strains were 99.90 and 99.98 %, respectively. Based on these results, we propose that Xuhuaishuia manganoxidans is a later heterotypic synonym of Brevirhabdus pacifica.
开展了多相分类学研究，以明确锰氧化许怀水菌模式菌株DY6-4T的确切分类地位。锰氧化许怀水菌DY6-4T的生理特性组合与太平洋短杆菌模式菌株22DY15T的生理特性一致。16S rRNA基因序列分析表明，锰氧化许怀水菌DY6-4T与太平洋短杆菌22DY15T的相似性为100%，并形成一个单系群。包括脂肪酸、醌和极性脂质在内的化学分类特征结果支持了这两个菌株之间的密切关系。这两个菌株之间的数字DNA-DNA杂交值和平均核苷酸同一性值分别为99.90%和99.98%。基于这些结果，我们提出锰氧化许怀水菌是太平洋短杆菌的晚出异名。
