Chlamydiae and Mycoplasma Laboratory, Institut Pasteur du Maroc, Casablanca, Morocco; Biochemistry and Immunology Laboratory, Faculty of Sciences, University Mohamed V Rabat, Morocco.
Cardiovascular Surgery Department, CHU Ibn Rochd, Casablanca, Morocco.
J Infect Public Health. 2018 Mar-Apr;11(2):246-249. doi: 10.1016/j.jiph.2017.07.029. Epub 2017 Aug 30.
Chlamydia pneumoniae is an intracellular bacterium responsible for respiratory diseases and is highly involved in cardiovascular disease development, mainly atherosclerosis. The main objective of our study was to evaluate C. pneumoniae prevalence in Moroccan patients suffering from cardiovascular diseases. A total of 115 patients with cardiovascular diseases were enrolled, and their clinical and behavioral information was recorded. Blood was sampled from all patients as well as the atheroma plaques from 36 patients undergoing surgery. Nested PCR was performed for C. pneumoniae DNA detection in both peripheral blood mononuclear cells (PBMCs) and atheroma plaques. Statistical analysis was performed using EpiInfo software. Data analysis showed cardiovascular disease dominance in men, with a sex ratio M/F of 3.4, a majority of tobacco users (52.2%), and many diabetics (44.3%). A significant difference between genders was shown for tobacco use (p<0.05). Positive cases for PBMCs and atheroma plaques were 61% and 86%, respectively, and a significant difference between PBMCs and atheroma plaque infection was identified (p=0.02). Data analysis also showed that 12% of patients presented only C. pneumoniae infection as a risk factor. Therefore, the high prevalence of C. pneumoniae suggests its involvement in atherosclerosis, and further investigation is recommended for confirmation.
肺炎衣原体是一种细胞内细菌，可导致呼吸道疾病，并高度参与心血管疾病的发展，主要是动脉粥样硬化。我们研究的主要目的是评估摩洛哥心血管疾病患者中肺炎衣原体的流行情况。共纳入 115 名心血管疾病患者，并记录了他们的临床和行为信息。采集所有患者的血液，以及 36 名接受手术的患者的动脉粥样硬化斑块。在周围血单核细胞 (PBMC) 和动脉粥样硬化斑块中均进行巢式 PCR 检测肺炎衣原体 DNA。使用 EpiInfo 软件进行统计分析。数据分析显示，心血管疾病在男性中占主导地位，男女比例为 3.4，多数为吸烟者（52.2%），许多糖尿病患者（44.3%）。吸烟情况在性别之间存在显著差异（p<0.05）。PBMC 和动脉粥样硬化斑块的阳性病例分别为 61%和 86%，且 PBMC 和动脉粥样硬化斑块感染之间存在显著差异（p=0.02）。数据分析还显示，12%的患者仅以肺炎衣原体感染作为危险因素。因此，肺炎衣原体的高流行率表明其与动脉粥样硬化有关，建议进一步调查以确认。
