Shaik Althaf Hussain, Shaik Shajidha Ruksar, Shaik Abdul Saheer, Daoud Ali, Salim Manoharadas, Kodidhela Lakshmi Devi
Central Laboratory, Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia.
Sri Adi Siva Sadguru Alli Saheb Sivaaryula Homeopathy Medical College, Guntakal, A.P., India.
Saudi J Biol Sci. 2021 Apr;28(4):2575-2580. doi: 10.1016/j.sjbs.2021.01.062. Epub 2021 Feb 10.
This research designed to analyze the and ameliorative action of maslinic acid (MA) and gallic acid (GA) on reactive oxygen species generating enzyme xanthine oxidase (XO) in isoprenaline or isoproterenol (ISO) induced myocardial infarcted rats.
Albino Wistar rats were categorized into four groups with eight rats in each group. A dose of 15 mg/kg of MA and GA were pretreated to each MA and GA groups for seven days. A dose of 85 mg/kg of ISO administered to the ISO group along with MA and GA groups except normal group on two consecutive days of pretreatment. All animals sacrificed and the heart tissues were collected for the analysis of XO. The molecular docking analysis of the compounds MA and GA with XO was analyzed by using Gold 3.0.1 software.
XO enzyme levels were significantly increased in the heart homogenate of ISO administered rats when compared to normal rats. Pretreatment of MA and GA to ISO treated rats significantly brought XO enzyme to the near normal levels which indicate the protective action of MA and GA against myocardial necrosis. The results were further supported by the molecular docking study which revealed the inhibition of XO enzyme by the formation of enzyme and ligand complex with the compounds MA and GA.
MA and GA compounds manifested the ameliorative effect against ISO administrated myocardial necrosis by inhibiting the free radical generating enzyme XO which is evidenced by both and studies.
本研究旨在分析齐墩果酸（MA）和没食子酸（GA）对异丙肾上腺素（ISO）诱导的心肌梗死大鼠中产生活性氧的酶黄嘌呤氧化酶（XO）的影响及改善作用。
将白化Wistar大鼠分为四组，每组八只。MA组和GA组分别给予15mg/kg的MA和GA预处理7天。除正常组外，ISO组在预处理的连续两天内与MA组和GA组一起给予85mg/kg的ISO。处死所有动物，收集心脏组织用于XO分析。使用Gold 3.0.1软件分析化合物MA和GA与XO的分子对接分析。
与正常大鼠相比，给予ISO的大鼠心脏匀浆中XO酶水平显著升高。对给予ISO的大鼠进行MA和GA预处理可使XO酶显著恢复至接近正常水平，这表明MA和GA对心肌坏死具有保护作用。分子对接研究进一步支持了该结果，该研究揭示了化合物MA和GA通过形成酶与配体复合物抑制XO酶。
MA和GA化合物通过抑制产生自由基的酶XO，对给予ISO的心肌坏死表现出改善作用，这在体外和体内研究中均得到证实。
