Department of Restorative Sciences and Biomaterials, Boston University Henry M. Goldman School of Dental Medicine, Boston, MA, USA.
Magnes Res. 2017 May 1;30(2):42-52. doi: 10.1684/mrh.2017.0422.
Biomaterials containing magnesium are used for implants and bone regeneration. However, mechanisms underlying the biologic effects of magnesium are still largely unknown and have not been examined on normal human osteoblasts. This study was designed to test the effect of supplemented Mg concentrations between 0.5 mM and 16 mM on the osteogenic behaviors of normal human primary osteoblasts. Human primary osteoblasts were cultured in the groups with various concentrations of supplemented magnesium for various time intervals. Cell proliferation was measured using crystal violet staining. Degree of Alkaline Phosphatase (ALP) activity was measured by fluorometric assay. Expression of osteocalcin was measured by immunosorbent assay. Mineralization of cultures was determined by Alizarin Red S staining. Results showed that initial cell attachment efficiency was not affected by supplemented Mg (P > 0.05). At 21 days, proliferation rates increased in groups containing 0.5 mM-4 mM supplemented Mg and decreased in groups of supplemented 8 mM and 16 mM Mg. ALP activity and osteocalcin expression were upregulated in groups of supplemented Mg between 0.5 mM-2.0 mM (P < 0.05), but downregulated in groups with supplemented Mg concentrations of 4mM and above (P < 0.05). Cultures with 1 mM and 2 mM supplemented Mg showed upregulated mineralization activity compared to the control (P < 0.05), but downregulated in groups with supplemented Mg concentrations of 4 mM and above (P < 0.05). The present study based on an experimental design demonstrated the impact of 2 mM supplemented Mg on induced-proliferation and differentiation of normal human osteoblasts.
生物材料中含有镁被用于植入物和骨再生。然而，镁的生物学效应的机制在很大程度上仍然未知，并且尚未在正常人类成骨细胞上进行检查。本研究旨在测试补充镁浓度在 0.5mM 和 16mM 之间对正常人类原代成骨细胞成骨行为的影响。将人类原代成骨细胞在具有各种补充镁浓度的组中培养不同的时间间隔。使用结晶紫染色测量细胞增殖。通过荧光测定法测量碱性磷酸酶（ALP）活性的程度。通过免疫吸附测定测量骨钙素的表达。通过茜素红 S 染色确定培养物的矿化。结果表明，初始细胞附着效率不受补充镁的影响（P>0.05）。在第 21 天，在含有 0.5mM-4mM 补充镁的组中增殖率增加，而在补充 8mM 和 16mM 镁的组中降低。在补充镁浓度在 0.5mM-2.0mM 之间的组中，ALP 活性和骨钙素表达上调（P<0.05），但在补充镁浓度为 4mM 及以上的组中下调（P<0.05）。与对照组相比，补充 1mM 和 2mM 镁的培养物显示出上调的矿化活性（P<0.05），但在补充镁浓度为 4mM 及以上的组中下调（P<0.05）。本研究基于实验设计，证明了补充 2mM 镁对正常人类成骨细胞的诱导增殖和分化的影响。
